Nucleotide sequence of a full length cDNA clone encoding for β-tubulin of the sea urchin Paracentrotus lividus

The authors have isolated from a lambda gt 10 cDNA library of P. lividus hatching blastula embryos several clones encoding for beta -tubulin. The DNA sequence of one such clone ( beta 2) containing a 1826 bp insert has been determined. The inferred polypeptide sequence shows that beta contains the c...

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Bibliographic Details
Published in:Nucleic acids research 1989-07, Vol.17 (14), p.5851-5851
Main Authors: DI BERNARDO, M. G, GIANGUZZA, F, CIACCIO, M, PALLA, F, COLOMBO, P, DI BLASI, F, FAIS, M, SPINELLI, G
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acids
Animals
Base Sequence
beta -tubulin
Biological and medical sciences
Blastocyst
cDNA
clones
DNA
DNA - genetics
Echinoidea
Embryo, Nonmammalian
Fundamental and applied biological sciences. Psychology
genes
Genes. Genome
Marine
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
nucleotide sequence
nucleotides
Paracentrotus lividus
proteins
Sea Urchins - embryology
Tubulin - genetics
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Summary:The authors have isolated from a lambda gt 10 cDNA library of P. lividus hatching blastula embryos several clones encoding for beta -tubulin. The DNA sequence of one such clone ( beta 2) containing a 1826 bp insert has been determined. The inferred polypeptide sequence shows that beta contains the coding information for 447 amino acids. Comparison with a human beta -tubulin polypeptide reveals amino acids identity greater than 96%. The amino acid substitutions and the insertions/deletions in the sea urchin beta 2-tubulin, most of which are clustered in the highly acidic C-terminal region of the protein, are indicated with one and three asterisks respectively.
ISSN:0305-1048
1362-4962