Rapid Covalent Ligation of Fluorescent Peptides to Water Solubilized Quantum Dots

Water solubilized nanoparticles such as CdSe−ZnS core−shell nanocrystals (quantum dots, QDs) have great potential in bioimaging and sensing applications due to their excellent photophysical properties. However, the efficient modification of QDs with complex biomolecules represents a significant chal...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2010-07, Vol.132 (29), p.10027-10033
Main Authors: Blanco-Canosa, Juan B, Medintz, Igor L, Farrell, Dorothy, Mattoussi, Hedi, Dawson, Philip E
Format: Article
Language:English
Subjects:
Animals
Benzaldehydes - chemistry
Cattle
Chymotrypsin - analysis
Drug Carriers - chemistry
Fluorescence Resonance Energy Transfer
Fluorescent Dyes - chemistry
Peptides - chemistry
Quantum Dots
Solubility
Spectrophotometry, Ultraviolet
Trypsin - analysis
Water - chemistry
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Summary:Water solubilized nanoparticles such as CdSe−ZnS core−shell nanocrystals (quantum dots, QDs) have great potential in bioimaging and sensing applications due to their excellent photophysical properties. However, the efficient modification of QDs with complex biomolecules represents a significant challenge. Here, we describe a straightforward arylhydrazone approach for the chemoselective covalent modification of QDs that is compatible with neutral pH and micromolar concentrations of the peptide target. The kinetics of covalent modification can be monitored spectroscopically at 354 nm in the presence of the QD and average peptide/QD ratios from 2:1 to 11:1 were achieved with excellent control over the desired valency. These results suggest that aniline catalyzed hydrazone ligation has the potencial to provide a general method for the controlled assembly of a variety of nanoparticle-biomolecule hybrids.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja910988d