Nucleotide sequence of a gene for indole-3-acetamide hydrolase from Bradyrhizobium japonicum

We have determined the nucleotide sequence of the bam gene, the genetic determinant of indole-3-acetamide hydrolase, an enzyme that catalyzes the conversion of indole-3-acetamide to indole-3-acetic acid in nitrogen-fixing bacterium Bradyrhizobium japonicum strain J1063. The sequence analysis indicat...

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Bibliographic Details
Published in:Nucleic acids research 1989-08, Vol.17 (15), p.6400-6400
Main Authors: SEKINE, M, WATANABE, K, SYONO, K
Format: Article
Language:English
Subjects:
Amidohydrolases - genetics
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Bradyrhizobium japonicum
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genes. Genome
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Rhizobiaceae - enzymology
Rhizobiaceae - genetics
Sequence Homology, Nucleic Acid
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Summary:We have determined the nucleotide sequence of the bam gene, the genetic determinant of indole-3-acetamide hydrolase, an enzyme that catalyzes the conversion of indole-3-acetamide to indole-3-acetic acid in nitrogen-fixing bacterium Bradyrhizobium japonicum strain J1063. The sequence analysis indicates that the bam locus contains an open reading frame of 465 amino acids which corresponds to a protein with a molecular weight of 50,266 daltons. High degree of homology were found among the central region of the putative products of the bam gene, the iaali gene from Pseudomonas savastanoi , and the tms2 gene from Agrobacterium tumefaciens .
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/17.15.6400