The development and characterization of protein-based stationary phases for studying drug–protein and protein–protein interactions

Protein-based liquid chromatography stationary phases are used in bioaffinity chromatography for studying drug–protein interactions, the determination of binding affinities, competitive and allosteric interactions, as well as for studying protein–protein interactions. This review addresses the devel...

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Bibliographic Details
Published in:Journal of Chromatography A 2011-12, Vol.1218 (49), p.8791-8798
Main Authors: Sanghvi, Mitesh, Moaddel, Ruin, Wainer, Irving W.
Format: Article
Language:English
Subjects:
affinity chromatography
binding capacity
ERR
Frontal affinity chromatography
heat shock proteins
HSA
Hsp90
Protein fishing
protein-protein interactions
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Summary:Protein-based liquid chromatography stationary phases are used in bioaffinity chromatography for studying drug–protein interactions, the determination of binding affinities, competitive and allosteric interactions, as well as for studying protein–protein interactions. This review addresses the development and characterization of protein-based stationary phase, and the application of these phases using frontal and zonal chromatography techniques. The approach will be illustrated using immobilized heat shock protein 90α and the immobilized estrogen related receptor stationary phases. In addition, the review discusses the use of the protein-coated magnetic beads for ligand and protein fishing as well as for the identification of unknown ligands from cellular or botanical extracts.
ISSN:0021-9673
1873-3778
DOI:10.1016/j.chroma.2011.05.067