Identification and phylogenetic analysis of Japanese Macaque Babesia-1 (JM-1) detected from a Japanese Macaque (Macaca fuscata fuscata)

We demonstrate here the identification and phylogenetic characterization of Babesia microti (B. microti)-like parasite detected from a splenectomized Japanese macaque (Macaca fuscata fuscata) at a facility for laboratory animal science. On Day 133 after splenectomy, intra-erythrocytic parasites were...

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Bibliographic Details
Published in:The American journal of tropical medicine and hygiene 2011-10, Vol.85 (4), p.635-638
Main Authors: Hirata, Haruyuki, Kawai, Satoru, Maeda, Mari, Jinnai, Michio, Fujisawa, Kohei, Katakai, Yuko, Hikosaka, Kenji, Tanabe, Kazuyuki, Yasutomi, Yasuhiro, Ishihara, Chiaki
Format: Article
Language:English
Subjects:
Animals
Babesia microti
Babesia microti - genetics
Babesia microti - isolation & purification
Base Sequence
DNA Primers
Female
Macaca
Macaca fuscata fuscata
Phylogeny
Polymerase Chain Reaction
Short Report
Splenectomy
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Summary:We demonstrate here the identification and phylogenetic characterization of Babesia microti (B. microti)-like parasite detected from a splenectomized Japanese macaque (Macaca fuscata fuscata) at a facility for laboratory animal science. On Day 133 after splenectomy, intra-erythrocytic parasites were found on light microscopic examination, and the level of parasitemia reached 0.3% on blood smear. Molecular characterization of the parasite using nested-polymerization chain reactions targeting the 18S rRNA, β-tubulin, and subunit 7 (eta) of the chaperonin-containing t-complex polypeptide 1 (CCT7) genes were identified as a B. microti-like parasite, designated the Japanese Macaque Babesia-1 (JM-1).
ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.2011.11-0035