Independent localization of MAP2, CaMKIIα and β-actin RNAs in low copy numbers

Messenger RNA localization involves the assembly of ribonucleoprotein particles (RNPs) and their subsequent transport along the cytoskeleton to their final destination. Here, we provide new evidence that microtubule‐associated protein 2 ( MAP2 ), calcium/calmodulin‐dependent protein kinase II ( CaMK...

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Bibliographic Details
Published in:EMBO reports 2011-10, Vol.12 (10), p.1077-1084
Main Authors: Mikl, Martin, Vendra, Georgia, Kiebler, Michael A
Format: Article
Language:English
Subjects:
Actin
Actins - genetics
Animals
Calcium-Calmodulin-Dependent Protein Kinase Type 2 - genetics
Calmodulin
CaMKIIα
Cytoskeleton
Dendrites - metabolism
EMBO27
EMBO36
Gene Expression Regulation
Kinases
Localization
MAP2 mRNA
Microtubule-Associated Proteins - genetics
mRNA
Neurons - metabolism
Proteins
Rats
Ribonucleoproteins - metabolism
RNA localization
RNA Transport
RNA, Messenger - metabolism
RNA-Binding Proteins - metabolism
Scientific Report
Scientific Reports
Staufen 2
β-actin
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Summary:Messenger RNA localization involves the assembly of ribonucleoprotein particles (RNPs) and their subsequent transport along the cytoskeleton to their final destination. Here, we provide new evidence that microtubule‐associated protein 2 ( MAP2 ), calcium/calmodulin‐dependent protein kinase II ( CaMKIIα ) and β‐actin RNAs localize to dendrites in distinct RNPs, which contain—unexpectedly—very few RNA molecules. The number of MAP2 molecules per particle is affected by synaptic activity and Staufen 2, indicating that RNP composition is tightly controlled. Our data suggest that the independent localization of individual RNAs in low copy numbers could contribute to tighter temporal and spatial control of expression in neurons and synapse‐specific plasticity. mRNA subcellular localization in neurons involves the formation of ribonucleoprotein particles (RNPs) that are transported through the cytoskeleton. The authors show that, unexpectedly, dendritic RNPs contain few molecules of mRNA and both the nature of these mRNAs and their number are tightly controlled.
ISSN:1469-221X
1469-3178
DOI:10.1038/embor.2011.149