Osmium tetroxide: a new probe for site-specific distortions in supercoiled DNAs

Supercoiled plasmids Col El and cDm 506 (a Col El derivative carrying the D. melanogaster histone gene repeat) were treated with 0s04 in presence of pyriaine and the reaction products were analyzed using different approaches. Gel electrophoresis showed that 0s04 binding to supercoiled DNA induced it...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 1984-02, Vol.12 (3), p.1725-1736
Main Authors: Glikin, Gerardo C., Vojtíškova, Marie, Rena-Descalzi, Liliana, Paleĉek, Emil
Format: Article
Language:English
Subjects:
Animals
Chemical Phenomena
Chemistry
DNA Restriction Enzymes
DNA, Single-Stranded - isolation & purification
DNA, Superhelical - genetics
Drosophila melanogaster
Drosophila melanogaster - genetics
Histones - genetics
Microscopy, Electron
Nucleic Acid Conformation
Nucleic Acid Denaturation
Osmium - pharmacology
Osmium Tetroxide - pharmacology
Plasmids - drug effects
Repetitive Sequences, Nucleic Acid
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Supercoiled plasmids Col El and cDm 506 (a Col El derivative carrying the D. melanogaster histone gene repeat) were treated with 0s04 in presence of pyriaine and the reaction products were analyzed using different approaches. Gel electrophoresis showed that 0s04 binding to supercoiled DNA induced its relaxation without nicking. The amount of osmium bound to DNA (as determined electrochernjcally) increased with the extent of DNA relaxation. As a result of osmium modification of supercoiled cDm 506, a single denaturation “bubble” was observed in the electron microscope. Mapping of the osmium binding site by S1 nuclease cleavage followed by restriction enzyme digestion has revealed one major site in the intergenic spacer between the H1 and H3 histone genes of D. melanogaster. This site differs from the site cleaved by S1 nuclease in supercoiled DNA in the absence of osmium.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/12.3.1725