Dimeric tRNA gene arrangement in Schizosaccharomyces pombe allows increased expression of the downstream gene

Three Schizosaccharomyces pombe dimeric tRNA genes, consisting of a tRNASer gene encoding a minor species with an intervening sequence followed by a tRNAMeti gene, have been described [Mao et al. (1980) Cell 21, 509–516: Hot-tinger et al. (1982) Mol. Gen. Genet. 188, 219–224; Willis et al. (1984) EM...

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Bibliographic Details
Published in:Nucleic acids research 1985-12, Vol.13 (24), p.8739-8747
Main Authors: Hottinger-Werlen, Andrea, Schaack, Jerome, Lapointe, Jacques, Mao, Jen-i, Nichols, Mark, Söll, Dieter
Format: Article
Language:English
Subjects:
Applied sciences
Cloning, Molecular
Exact sciences and technology
Gene Expression Regulation
Genes, Fungal
Genetic Linkage
Methionine
Other techniques and industries
RNA, Fungal - genetics
RNA, Transfer - genetics
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomycetales - genetics
Schizosaccharomyces - genetics
Schizosaccharomyces pombe
Serine
Suppression, Genetic
Transcription, Genetic
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Summary:Three Schizosaccharomyces pombe dimeric tRNA genes, consisting of a tRNASer gene encoding a minor species with an intervening sequence followed by a tRNAMeti gene, have been described [Mao et al. (1980) Cell 21, 509–516: Hot-tinger et al. (1982) Mol. Gen. Genet. 188, 219–224; Willis et al. (1984) EMBO J. 3, l573–1580]. We have examined the reason for the dimeric structure by comparing the transcriptional efficiencies and competitive abilities of the genes subcloned from the dimeric arrangement. Both of the subcloned genes are active in vivo in Saccharomyces cerevisiae, but only the tRNASer gene is efficiently transcribed in vitro. The tRNAMeti gene competes efficiently for transcription factors, while the tRNAMeti gene does so only weakly. Thus, it appears that the dimeric arrangement is required to support expression of the tRNAMeti gene. S. pombe genes encoding major species of tRNASer are transcribed considerably less efficiently than are the minor genes from the dimers, so coupling of the tRNAMeti gene to the minor species genes should lead to efficient production of tRNAMeti.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/13.24.8739