Comparison of an automated repetitive sequence-based PCR microbial typing system with IS6110-restriction fragment length polymorphism for epidemiologic investigation of clinical Mycobacterium tuberculosis isolates in Korea

• Published in: Annals of laboratory medicine 2011, 31(4), , pp.282-284
• Main Authors: Jang, Mi Hee, Choi, Go Eun, Shin, Bo-Moon, Lee, Seon Ho, Kim, Sung-Ryul, Chang, Chulhun L, Kim, Jeong-Man
• Format: Article
• Language: English
• Subjects: Automation; Bacterial Typing Techniques; Brief Communication; Epidemiologic Methods; Genotype; Humans; Mycobacterium tuberculosis - classification; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis - isolation & purification; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Reagent Kits, Diagnostic; Repetitive Sequences, Nucleic Acid; Republic of Korea - epidemiology; Tuberculosis - diagnosis; Tuberculosis - epidemiology; Tuberculosis - microbiology; 병리학
• ISSN: 1598-6535; 2234-3806; 2234-3814
• DOI: 10.3343/kjlm.2011.31.4.282

Tuberculosis remains a severe public health problem worldwide. Presently, genotyping is used for conducting epidemiologic and clinical studies on tuberculosis cases. We evaluated the efficacy of the repetitive sequence-based PCR (rep-PCR)-based DiversiLab™ system (bioMérieux, France) over the IS6110-restriction fragment length polymorphism analysis for detecting Mycobacterium tuberculosis. In all, 89 clinical M. tuberculosis isolates collected nationwide from Korea were used. The DiversiLab system allocated the 89 isolates to 8 groups with 1 unique isolate when a similarity level of 95% was applied. Seventy-six isolates of the Beijing family and 13 isolates of non-Beijing family strains were irregularly distributed regardless of rep-PCR groups. The DiversiLab system generated a rapid, sensitive, and standardized result. It can be used to conduct molecular epidemiologic studies to identify clinical M. tuberculosis isolates in Korea.