Genome-wide analysis of OCT4 binding sites in glioblastoma cancer cells

OCT4, a member of the POU family of gene products, is an octamer motif-binding transcription factor. As it is known to play a crucial role in cancer processes including proliferation, invasion, and chemoradioresistance, it is important to identify the direct targets of OCT4 in living cancer cells. H...

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Bibliographic Details
Published in:Journal of Zhejiang University. B. Science 2011-10, Vol.12 (10), p.812-819
Main Authors: Fang, Xue-feng, Zhang, Wei-yi, Zhao, Na, Yu, Wei, Ding, Dong, Hong, Xu, Li, Li-sha, Zhang, Hua-rong, Zheng, Shu, Lin, Biao-yang
Format: Article
Language:English
Subjects:
Antibody Specificity
Binding Sites
Biomedical and Life Sciences
Biomedicine
Brain Neoplasms - etiology
Brain Neoplasms - genetics
Cancer
Cell Line, Tumor
Chromatin Immunoprecipitation
Genome, Human
Glioblastoma - etiology
Glioblastoma - genetics
Humans
Octamer Transcription Factor-3 - physiology
Transcription Factors - metabolism
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Summary:OCT4, a member of the POU family of gene products, is an octamer motif-binding transcription factor. As it is known to play a crucial role in cancer processes including proliferation, invasion, and chemoradioresistance, it is important to identify the direct targets of OCT4 in living cancer cells. Here, chromatin immunoprecipitation-sequencing （ChlP-seq） was used to identify OCT4 binding sites in glioblastoma cancer cells. The results showed that 5438 OCT4 binding sites were localized in the glioblastoma cancer genome and that these sites contained a consensus sequence TTTkswTw （k=T or G, s=C or G, w=A or T）, which occurred 3931 times in 2312 OCT4 binding regions. Furthermore, binding motifs of some other transcription factors were identified in OCT4 binding regions. Our results provide a valuable dataset for understanding gene regulation mechanisms underlying the function of OCT4 in glioblastoma cancer.
ISSN:1673-1581
1862-1783
DOI:10.1631/jzus.B1100059