Arabidopsis CSLD1 and CSLD4 are required for cellulose deposition and normal growth of pollen tubes

• Published in: Journal of experimental botany 2011-10, Vol.62 (14), p.5161-5177
• Main Authors: Wang, Wei, Wang, Li, Chen, Chen, Xiong, Guangyan, Tan, Xiao-Yun, Yang, Ke-Zhen, Wang, Zi-Chen, Zhou, Yihua, De Ye, Chen, Li-Qun
• Format: Article
• Language: English
• Subjects: Arabidopsis; Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - metabolism; Arabidopsis Proteins; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Biological and medical sciences; Cell growth; Cell membranes; Cell walls; cellulose; Cellulose - metabolism; Fluorescence; Fundamental and applied biological sciences. Psychology; Gametophytes; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; genes; genetics; Germination; Glucosyltransferases; Glucosyltransferases - genetics; Glucosyltransferases - metabolism; Golgi apparatus; growth & development; metabolism; mutants; mutation; phenotype; Plant cells; Plant physiology and development; Plants; plasma membrane; Pollen; Pollen - genetics; Pollen - metabolism; Pollen Tube; Pollen Tube - genetics; Pollen Tube - growth & development; Pollen Tube - metabolism; Pollen tubes; proteins; RESEARCH PAPER; Research Papers; Sexual reproduction; Vegetative and sexual reproduction, floral biology, fructification
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jxb/err221

The cell wall is important for pollen tube growth, but little is known about the molecular mechanism that controls cell wall deposition in pollen tubes. Here, the functional characterization of the pollen-expressed Arabidopsis cellulose synthase-like D genes CSLD1 and CSLD4 that are required for pollen tube growth is reported. Both CSLD1 and CSLD4 are highly expressed in mature pollen grains and pollen tubes. The CSLD1 and CSLD4 proteins are located in the Golgi apparatus and transported to the plasma membrane of the tip region of growing pollen tubes, where cellulose is actively synthesized. Mutations in CSLD1 and CSLD4 caused a significant reduction in cellulose deposition in the pollen tube wall and a remarkable disorganization of the pollen tube wall layers, which disrupted the genetic transmission of the male gametophyte. In csld1 and csld4 single mutants and in the csld1 csld4 double mutant, all the mutant pollen tubes exhibited similar phenotypes: the pollen tubes grew extremely abnormally both in vitro and in vivo, which indicates that CSLD1 and CSLD4 are not functionally redundant. Taken together, these results suggest that CSLD1 and CSLD4 play important roles in pollen tube growth, probably through participation in cellulose synthesis of the pollen tube wall.