'Bioluminescent' reporter phage for the detection of Category A bacterial pathogens

• Published in: Journal of visualized experiments 2011-07 (53), p.e2740-e2740
• Main Authors: Schofield, David A, Molineux, Ian J, Westwater, Caroline
• Format: Article
• Language: English
• Subjects: Anthrax - diagnosis; Anthrax - microbiology; Bacillus anthracis - isolation & purification; Bacillus anthracis - virology; Bacterial Proteins - analysis; Bacterial Proteins - genetics; Bacteriophages - chemistry; Bacteriophages - enzymology; Bacteriophages - genetics; Immunology; Luciferases - analysis; Luciferases - genetics; Luminescent Measurements - methods; Plague - diagnosis; Plague - microbiology; Vibrio - enzymology; Vibrio - genetics; Yersinia pestis - isolation & purification; Yersinia pestis - virology
• ISSN: 1940-087X; 1940-087X
• DOI: 10.3791/2740

Yersinia pestis and Bacillus anthracis are Category A bacterial pathogens that are the causative agents of the plague and anthrax, respectively. Although the natural occurrence of both diseases' is now relatively rare, the possibility of terrorist groups using these pathogens as a bioweapon is real. Because of the disease's inherent communicability, rapid clinical course, and high mortality rate, it is critical that an outbreak be detected quickly. Therefore methodologies that provide rapid detection and diagnosis are essential to ensure immediate implementation of public health measures and activation of crisis management. Recombinant reporter phage may provide a rapid and specific approach for the detection of Y. pestis and B. anthracis. The Centers for Disease Control and Prevention currently use the classical phage lysis assays for the confirmed identification of these bacterial pathogens. These assays take advantage of naturally occurring phage which are specific and lytic for their bacterial hosts. After overnight growth of the cultivated bacterium in the presence of the specific phage, the formation of plaques (bacterial lysis) provides a positive identification of the bacterial target. Although these assays are robust, they suffer from three shortcomings: 1) they are laboratory based; 2) they require bacterial isolation and cultivation from the suspected sample, and 3) they take 24-36 h to complete. To address these issues, recombinant "light-tagged" reporter phage were genetically engineered by integrating the Vibrio harveyi luxAB genes into the genome of Y. pestis and B. anthracis specific phage. The resulting luxAB reporter phage were able to detect their specific target by rapidly (within minutes) and sensitively conferring a bioluminescent phenotype to recipient cells. Importantly, detection was obtained either with cultivated recipient cells or with mock-infected clinical specimens. For demonstration purposes, here we describe the method for the phage-mediated detection of a known Y. pestis isolate using a luxAB reporter phage constructed from the CDC plague diagnostic phage ΦA1122 (Figure 1). A similar method, with minor modifications (e.g. change in growth temperature and media), may be used for the detection of B. anthracis isolates using the B. anthracis reporter phage Wβ::luxAB. The method describes the phage-mediated transduction of a biolumescent phenotype to cultivated Y. pestis cells which are subsequently measured using a microplate