Microarray-Based Multiplexed Scanometric Immunoassay for Protein Cancer Markers Using Gold Nanoparticle Probes

We report the use of electroless gold deposition as a light scattering signal enhancer in a multiplexed, microarray-based scanometric immunoassay using gold nanoparticle probes. The use of gold development results in greater signal enhancement than the typical silver development, and multiple rounds...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2009-11, Vol.81 (21), p.9183-9187
Main Authors: Kim, Dongwoo, Daniel, Weston L, Mirkin, Chad A
Format: Article
Language:English
Subjects:
alpha-Fetoproteins - analysis
Analytical chemistry
Antibodies, Immobilized - immunology
Antibodies, Immobilized - metabolism
Antigens
Cancer
Chemistry
Chorionic Gonadotropin - analysis
Exact sciences and technology
General, instrumentation
Gold
Gold - chemistry
Humans
Immunoassay
Immunoassay - methods
Male
Metal Nanoparticles - chemistry
Miscellaneous
Nanoparticles
Prostate-Specific Antigen - analysis
Prostate-Specific Antigen - blood
Protein Array Analysis
Proteins
Proteins - analysis
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Summary:We report the use of electroless gold deposition as a light scattering signal enhancer in a multiplexed, microarray-based scanometric immunoassay using gold nanoparticle probes. The use of gold development results in greater signal enhancement than the typical silver development, and multiple rounds of metal development were found to increase the resulting signal compared to one development. Using these conditions, the assay was capable of detecting 300 aM (∼9000 copies) of prostate specific antigen in buffer and 3 fM in 10% serum. Additionally, the highly selective detection of three protein cancer markers at low picomolar concentrations in buffer and 10% serum was demonstrated. The use of gold deposition may have significant utility in scanometric detection schemes and broader clinical and research applications.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac9018389