Evaluation of the Botanical Authenticity and Phytochemical Profile of Black Cohosh Products by High-Performance Liquid Chromatography with Selected Ion Monitoring Liquid Chromatography−Mass Spectrometry

• Published in: Journal of agricultural and food chemistry 2006-05, Vol.54 (9), p.3242-3253
• Main Authors: Jiang, Bei, Kronenberg, Fredi, Nuntanakorn, Paiboon, Qiu, Ming-Hua, Kennelly, Edward J
• Format: Article
• Language: English
• Subjects: Actaea racemosa; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Cimicifuga - chemistry; dietary supplements; Drug Contamination; Feeding. Feeding behavior; Food industries; formononetin; Fundamental and applied biological sciences. Psychology; General aspects; glycosides; Glycosides - analysis; high performance liquid chromatography; Isoflavones - analysis; liquid chromatography; mass spectrometry; Mass Spectrometry - methods; Methods of analysis, processing and quality control, regulation, standards; phenolic compounds; Phenols - analysis; product authenticity; Reproducibility of Results; selected ion monitoring liquid chromatography; triterpene glycosides; Triterpenes - analysis; triterpenoids; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf0606149

Black cohosh (Actaea racemosa L., syn. Cimicifuga racemosa L.) has become increasingly popular as a dietary supplement in the United States for the treatment of symptoms related to menopause, but the botanical authenticity of most products containing black cohosh has not been evaluated, nor is manufacturing highly regulated in the United States. In this study, 11 black cohosh products were analyzed for triterpene glycosides, phenolic constituents, and formononetin by high-performance liquid chromatography−photodiode array detection and a new selected ion monitoring liquid chromatography−mass spectrometry method. Three of the 11 products were found to contain the marker compound cimifugin and not cimiracemoside C, thereby indicating that these plants contain Asian Actaea instead of black cohosh. One product contained both black cohosh and an Asian Actaea species. For the products containing only black cohosh, there was significant product-to-product variability in the amounts of the selected triterpene glycosides and phenolic constituents, and as expected, no formononetin was detected. Keywords: Black cohosh; dietary supplements; quality control; triterpene glycosides; phenolic constituents; formononetin; Actaea racemosa; Cimicifuga racemosa; Asian Actaea species