A comparison of experimental and theoretical melting maps for replicative form of φX174 DNA

A previously elaborated technique for fixing a chosen partially melted state of DHA with glyoxal was used in a study of the melting process of the replicative form (RF III) of φX174DNA. Electron-microscopic maps corresponding to five points of the melting curve of RF III were obtained and compared w...

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Bibliographic Details
Published in:Nucleic acids research 1982-03, Vol.10 (6), p.1867-1876
Main Authors: Lyubchenko, Yu.L., Kalambet, Yu.A., Lyamichev, V.I., Borovik, A.S.
Format: Article
Language:English
Subjects:
Bacteriophage phi X 174 - genetics
DNA Replication
Microscopy, Electron
Models, Genetic
Nucleic Acid Conformation
Nucleic Acid Denaturation
phage Phi X174
Virus Replication
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Summary:A previously elaborated technique for fixing a chosen partially melted state of DHA with glyoxal was used in a study of the melting process of the replicative form (RF III) of φX174DNA. Electron-microscopic maps corresponding to five points of the melting curve of RF III were obtained and compared with the theoretical melting maps obtained in (4) and (6). Ibis comparison clearly shows that only rigorous calculations (4) and not the ones proposed by Azbel (6,7) correctly predict the course of RP III melting.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/10.6.1867