Carbodiimide modification analysis of aminoacylated yeast phenylalanine tRNA: evidence for change in the apex region

The G- and U-specific reagent, carbodiimide was used to probe the solution structure of aminoacylated yeast phenylalanine tRNA. Both quantitative and qualitative changes in modification were observed when the modification patterns of tRNA-CCA(3'OH), tRNA-CCA(3'NH2) and phe-tRNA-CCA(3'...

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Bibliographic Details
Published in:Nucleic acids research 1982-04, Vol.10 (7), p.2419-2437
Main Authors: Fritzinger, D C, Fournier, M J
Format: Article
Language:English
Subjects:
Carbodiimides
Chemical Phenomena
Chemistry
CME-Carbodiimide - analogs & derivatives
Deoxyadenosines - analogs & derivatives
Models, Molecular
Nucleic Acid Conformation
Phosphorus Radioisotopes
RNA, Transfer, Amino Acyl
Saccharomyces cerevisiae
Structure-Activity Relationship
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Summary:The G- and U-specific reagent, carbodiimide was used to probe the solution structure of aminoacylated yeast phenylalanine tRNA. Both quantitative and qualitative changes in modification were observed when the modification patterns of tRNA-CCA(3'OH), tRNA-CCA(3'NH2) and phe-tRNA-CCA(3'NH2) were compared. Five nucleotides were modified in all cases, D16 and G20 in the D-loop, U33 and Gm34 in the anticodon loop and U47, in the region of the extra arm. Small changes occurred in the D-loop with incorporation of the adenosine analogue manifest as new, low levels of modification of G22 (D-stem) and a loss of sensitivity to Mg+2 in modification of D16. Aminoacylation resulted in new modification of G19, modification of a residue in the T psi CG sequence, and a 2.5-fold increase in modification of G22. Taken together the results show that aminoacylation causes increased exposure of bases in the apex region of the L-shaped molecule where the D- and psi-loops are joined. The effects observed could occur as a consequence of stable or dynamic changes in conformation.
ISSN:0305-1048
1362-4962