The influence of elasticity and surface roughness on myogenic and osteogenic-differentiation of cells on silk-elastin biomaterials

Abstract The interactions of C2C12 myoblasts and human bone marrow stem cells (hMSCs) with silk-tropoelastin biomaterials, and the capacity of each to promote attachment, proliferation, and either myogenic- or osteogenic-differentiation were investigated. Temperature-controlled water vapor annealing...

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Bibliographic Details
Published in:Biomaterials 2011-12, Vol.32 (34), p.8979-8989
Main Authors: Hu, Xiao, Park, Sang-Hyug, Gil, Eun Seok, Xia, Xiao-Xia, Weiss, Anthony S, Kaplan, David L
Format: Article
Language:English
Subjects:
Adult
Advanced Basic Science
Animals
Biocompatible Materials - chemistry
Biomaterials
Bombyx - chemistry
Cell Adhesion
Cell Differentiation
Cell Line
Cells, Cultured
Dentistry
Elasticity
Elastin
Hematopoietic Stem Cells - cytology
Humans
Male
Muscle cells
Myoblasts - cytology
Osteogenesis
Silk
Silk - chemistry
Stem cells
Surface Properties
Tropoelastin - chemistry
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Summary:Abstract The interactions of C2C12 myoblasts and human bone marrow stem cells (hMSCs) with silk-tropoelastin biomaterials, and the capacity of each to promote attachment, proliferation, and either myogenic- or osteogenic-differentiation were investigated. Temperature-controlled water vapor annealing was used to control beta-sheet crystal formation to generate insoluble silk-tropoelastin biomaterial matrices at defined ratios of the two proteins. These ratios controlled surface roughness and micro/nano-scale topological patterns, and elastic modulus, stiffness, yield stress, and tensile strength. A combination of low surface roughness and high stiffness in the silk-tropoelastin materials promoted proliferation and myogenic-differentiation of C2C12 cells. In contrast, high surface roughness with micro/nano-scale surface patterns was favored by hMSCs. Increasing the content of human tropoelastin in the silk-tropoelastin materials enhanced the proliferation and osteogenic-differentiation of hMSCs. We conclude that the silk-tropoelastin composition facilitates fine tuning of the growth and differentiation of these cells.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2011.08.037