Differential Function of N-Cadherin and Cadherin-7 in the Control of Embryonic Cell Motility

• Published in: The Journal of cell biology 1999-07, Vol.146 (2), p.501-516
• Main Authors: Dufour, Sylvie, Beauvais-Jouneau, Alice, Delouvée, Annie, Thiery, Jean Paul
• Format: Article
• Language: English
• Subjects: Animals; beta Catenin; Cadherins; Cadherins - genetics; Cadherins - metabolism; Cell Adhesion; Cell Adhesion Molecules - metabolism; Cell aggregates; Cell Aggregation; Cell motility; Cell Movement; Cell Size; Cell Transplantation; Cells; Cellular biology; Chick Embryo; Cycloheximide - pharmacology; Cytoskeletal Proteins - metabolism; Down-Regulation - drug effects; Embryonic cells; Embryos; Extracellular Matrix Proteins - metabolism; Fibronectins - metabolism; Focal Adhesion Kinase 1; Focal Adhesion Protein-Tyrosine Kinases; Integrins; Integrins - metabolism; L cells; Life Sciences; Mice; Microscopy, Video; Neurons; Original; Phosphorylation; Protein-Tyrosine Kinases - metabolism; Signal Transduction; Trans-Activators; Transfection; Tumor Cells, Cultured
• ISSN: 0021-9525; 1540-8140
• DOI: 10.1083/jcb.146.2.501

Similar amounts of N-cadherin and cadherin-7, the prototypes of type I and type II cadherin, induced cell-cell adhesion in murine sarcoma 180 transfectants, Ncad-1 and cad7-29, respectively. However, in the initial phase of aggregation, Ncad-1 cells aggregated more rapidly than cad7-29 cells. Isolated Ncad-1 and cad7-29 cells adhered and spread in a similar manner on fibronectin (FN), whereas aggregated cad7-29 cells were more motile and dispersed than aggregated Ncad-1 cells. cad7-29 cells established transient contacts with their neighbors which were stabilized if FN-cell interactions were perturbed. In contrast, Ncad-1 cells remained in close contact when they migrated on FN. Both β-catenin and cadherin were more rapidly down-regulated in cad7-29 than in Ncad-1 cells treated with cycloheximide, suggesting a higher turnover rate for cadherin-7-mediated cell-cell contacts than for those mediated by N-cadherin. The extent of FN-dependent focal adhesion kinase phosphorylation was much lower if the cells had initiated N-cadherin-mediated rather than cadherin-7-mediated cell adhesion before plating. On grafting into the embryo, Ncad-1 cells did not migrate and remained at or close to the graft site, even after 48 h, whereas grafted cad7-29 cells dispersed efficiently into embryonic structures. Thus, the adhesive phenotype of cadherin-7-expressing cells is regulated by the nature of the extracellular matrix environment which also controls the migratory behavior of the cells. In addition, adhesions mediated by different cadherins differentially regulate FN-dependent signaling. The transient contacts specifically observed in cadherin-7-expressing cells may also be important in the control of cell motility.