Lysine tRNAs from Bacillus subtilis 168: function of the isoacceptors in a rabbit reticulocyte cell-free protein-synthesizing system

The two principal tRNALys isoaccepting species of Bacillus subtilis were compared in their functional activity in translating rabbit globin. Although neither species demonstrates any preference in reading either of the lysine codons, there is an overall preference for tRNA3Lys in lysine incorporatio...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 1982-05, Vol.10 (10), p.3117-3123
Main Authors: Smith, David W.E., McNamara, Anne L., Vold, Barbara S.
Format: Article
Language:English
Subjects:
Animals
Bacillus subtilis
Bacillus subtilis - genetics
Globins - genetics
Macromolecular Substances
Protein Biosynthesis
Rabbits
Reticulocytes - metabolism
RNA, Transfer, Amino Acyl - genetics
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The two principal tRNALys isoaccepting species of Bacillus subtilis were compared in their functional activity in translating rabbit globin. Although neither species demonstrates any preference in reading either of the lysine codons, there is an overall preference for tRNA3Lys in lysine incorporation. The ratios of lysine incorporated by the two species into the different lysine-containing sites in the globin subunits vary over a more than two-fold range. As described in the accompanying paper, tRNA1Lys is a hypomodified form of tRNA3Lys. Consistent with studies on other tRNA species, the fully modified isoacceptor functions preferentially. In contrast to these results, however, the fully modified isoacceptor (tRNA3Lys) is found predominantly in rapidly dividing cells while the hypomodified isoacceptor (tRNA1Lys) predominates in the stationary cells and spores of B. subtilis.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/10.10.3117