Efficacy of polymeric encapsulated C5a peptidase–based group B streptococcus vaccines in a murine model

Objective The purpose was to examine in mice the efficacy of various polymeric-encapsulated C5a peptidase vaccine formulations in eliciting a long-term immune response and preventing group B streptococcus (GBS) infection. Study Design C5a peptidase was encapsulated in semipermeable microspheres of p...

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Bibliographic Details
Published in:American journal of obstetrics and gynecology 2011-09, Vol.205 (3), p.249.e1-249.e8
Main Authors: Santillan, Donna A., PhD, Rai, Karishma K., MD, Santillan, Mark K., MD, Krishnamachari, Yogita, PhD, Salem, Aliasger K., PhD, Hunter, Stephen K., MD, PhD
Format: Article
Language:English
Subjects:
Adhesins, Bacterial - immunology
Animals
antigen encapsulation
Biological and medical sciences
C5a peptidase
Endopeptidases - immunology
Female
group B streptococcus
Gynecology. Andrology. Obstetrics
Medical sciences
Mice
Mice, Inbred ICR
microparticle
microsphere
Microspheres
mouse
Obstetrics and Gynecology
poly(lactide-coglycolide)
Streptococcal Infections - prevention & control
Streptococcal Vaccines - immunology
Streptococcus agalactiae - immunology
vaccine
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Summary:Objective The purpose was to examine in mice the efficacy of various polymeric-encapsulated C5a peptidase vaccine formulations in eliciting a long-term immune response and preventing group B streptococcus (GBS) infection. Study Design C5a peptidase was encapsulated in semipermeable microspheres of poly(lactide-coglycolide) (PLGA). Female ICR mice were immunized with 0, 10, or 30 μg of encapsulated C5a peptidase within 2 different formulations of PLGA polymers. Booster doses were given at weeks 4 and 8. Antibody responses were measured by enzyme-linked immunosorbent assay at weeks 4, 8, 11, and 40. Vaginal challenges with GBS types 1a, III, and V were performed at week 12. Results Thirty microgram doses of the 75:25 and 50:50 PLGA formulations generate the highest and most sustained C5a peptidase–specific immune responses. Mice that received encapsulated C5a peptidase were significantly protected from vaginal colonization compared with mice that received empty microspheres. Conclusion Encapsulated C5a peptidase elicited significant immune responses and protection against a GBS challenge. C5a peptidase microsphere encapsulation has potential as a GBS vaccine.
ISSN:0002-9378
1097-6868
DOI:10.1016/j.ajog.2011.06.024