Purification and characterization of a NADPH-dependent aldehyde reductase from mung bean that detoxifies eutypine, a toxin from Eutypa lata

Eutypine (4-hydroxy-3-[3-methyl-3-butene-1-ynyl] benzaldehyde) is a toxin produced by Eutypa lata, the causal agent of eutypa dieback in the grapevine (Vitis vinifera). Eutypine isenzymatically converted by numerous plant tissues into eutypinol (4-hydroxy-3-[3-methyl-3-butene-1-ynyl] benzyl alcohol)...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1999-02, Vol.119 (2), p.621-626
Main Authors: Colrat, S, Latche, A, Guis, M, Pech, J.C, Bouzayen, M, Fallot, J, Roustan, J.P
Format: Article
Language:English
Subjects:
Alcohols
aldehyde oxidoreductases
Aldehydes
Biochemistry and Macromolecular Structure
Biological and medical sciences
Dehydrogenases
Disease resistance
enzyme activity
Enzymes
etiolation
Eutypa
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
Hypocotyls
metabolic detoxification
metabolites
molecular weight
mycotoxins
Pathology, epidemiology, host-fungus relationships. Damages, economic importance
Phytopathology. Animal pests. Plant and forest protection
phytotoxicity
Plant diseases
Plants
Substrate specificity
substrates
Toxins
Vigna radiata
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Summary:Eutypine (4-hydroxy-3-[3-methyl-3-butene-1-ynyl] benzaldehyde) is a toxin produced by Eutypa lata, the causal agent of eutypa dieback in the grapevine (Vitis vinifera). Eutypine isenzymatically converted by numerous plant tissues into eutypinol (4-hydroxy-3-[3-methyl-3-butene-1-ynyl] benzyl alcohol), a metabolite that is nontoxic to grapevine. We report a four-step procedure for the purification to apparent electrophoretic homogeneity of a eutypine-reducing enzyme (ERE) from etiolated mung bean (Vigna radiata) hypocotyls. The purified protein is a monomer of 36 kD, uses NADPH as a cofactor, and exhibits a Km value of 6.3 micromolars for eutypine and a high affinity for 3- and 4-nitro-benzaldehyde. The enzyme failed to catalyze the reverse reaction using eutypinol as a substrate. ERE detoxifies eutypine efficiently over a pH range from 6.2 to 7.5. These data strongly suggest that ERE is an aldehyde reductase that could probably be classified into the aldo-keto reductase superfamily. We discuss the possible role of this enzyme in eutypine detoxification.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.119.2.621