Microcantilevers modified by specific peptide for selective detection of trimethylamine

We report a biosensor based on a microcantilever that is modified by a specific peptide for highly selective detection of trimethylamine (TMA). The assay is based on binding-induced bending of the peptide functionalized microcantilevers. The sensor is selectively responsive to TMA. The amplitude of...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2011-12, Vol.30 (1), p.140-144
Main Authors: Huang, Xin, Li, Mingfu, Xu, Xiaohe, Chen, Hongjun, Ji, Hai-Feng, Zhu, Shuifang
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensor
Biosensors
Biotechnology
Equipment Design
Equipment Failure Analysis
Food Analysis - instrumentation
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Methylamines - analysis
Micro-Electrical-Mechanical Systems - instrumentation
Microcantilever
Peptides - chemistry
Protein Interaction Mapping - instrumentation
Trimethylamine
Various methods and equipments
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Summary:We report a biosensor based on a microcantilever that is modified by a specific peptide for highly selective detection of trimethylamine (TMA). The assay is based on binding-induced bending of the peptide functionalized microcantilevers. The sensor is selectively responsive to TMA. The amplitude of microcantilever bending at equilibrium is a function of the concentration of TMA with a dynamic range from 8 ppm to 800 ppm. The detection limit is approximately 8 ppm. There is a good intra-sensor and an acceptable inter-sensor reproducibility as evidenced by the standard deviation of 5% and 15%, respectively.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2011.09.001