Quantitative and phenotypic analysis of bone marrow-derived cells in the intact and inflamed central nervous system

Bone marrow has been proposed as a possible source of cells capable of replacing injured neural cells in diseases such as Multiple Sclerosis (MS). Previous studies have reported conflicting results regarding the transformation of bone marrow cells into neural cells in vivo. This study is a detailed...

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Bibliographic Details
Published in:Cell adhesion & migration 2011-09, Vol.5 (5), p.373-381
Main Authors: Short, Martin A, Campanale, Naomi, Litwak, Sara, Bernard, Claude C A
Format: Article
Language:English
Subjects:
Animals
Binding
Biology
Biomarkers - metabolism
Bioscience
Bone Marrow - metabolism
Bone Marrow Cells - metabolism
Bone Marrow Cells - pathology
Bone Marrow Transplantation
Calcium
Cancer
Cell
Central Nervous System - metabolism
Central Nervous System - pathology
Cycle
Disease Models, Animal
Encephalomyelitis, Autoimmune, Experimental - metabolism
Encephalomyelitis, Autoimmune, Experimental - pathology
Gangliosides - metabolism
Green Fluorescent Proteins - metabolism
Landes
Leukocyte Common Antigens - metabolism
Mice
Mice, Inbred C57BL
Neurons - metabolism
Organogenesis
Phenotype
Proteins
Short Communication
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Summary:Bone marrow has been proposed as a possible source of cells capable of replacing injured neural cells in diseases such as Multiple Sclerosis (MS). Previous studies have reported conflicting results regarding the transformation of bone marrow cells into neural cells in vivo. This study is a detailed analysis of the fate of bone marrow derived cells (BMDC) in the CNS of C57Bl/6 mice with and without experimental autoimmune encephalomyelitis using flow cytometry to identify GFP-labeled BMDC that lacked the pan-hematopoietic marker, CD45 and co-expressed neural markers polysialic acid-neural cell adhesion molecule or A2B5. A small number of BMDC displaying neural markers and lacking CD45 expression was identified within both the non-inflamed and inflamed CNS. However, the majority of BMDC exhibited a hematopoietic phenotype.
ISSN:1933-6918
1933-6926
DOI:10.4161/cam.5.5.17948