Isolation and molecular characterisation of Mycobacterium bovis from raw milk in Tunisia

Background: Consumption of raw milk and unpasteurized dairy products is common in Tunisia where bovine tuberculosis remains enzootic. We herein investigated the frequency of M. bovis isolation from raw milk. Methods: Three hundred and six milk samples collected from 102 infected cows in different Tu...

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Bibliographic Details
Published in:African health sciences 2011-08, Vol.11 (3), p.S2-S5
Main Authors: Kahla, I Ben, Boschiroli, ML, Souissi, F, Cherif, N, Benzarti, M, Boukadida, J, Hammami, S
Format: Article
Language:English
Subjects:
Animals
Bacteria
Cattle
Consumers
Dairy products
Genotype
Genotypes
Health and Medicine
Humans
Infection
Infections
Milk
Milk - microbiology
Mycobacterium bovis
Mycobacterium bovis - genetics
Mycobacterium bovis - isolation & purification
Raw milk, Zoonosis
Risk factors
spoligotyping
Tuberculosis
Tunisia
Zoonoses
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Summary:Background: Consumption of raw milk and unpasteurized dairy products is common in Tunisia where bovine tuberculosis remains enzootic. We herein investigated the frequency of M. bovis isolation from raw milk. Methods: Three hundred and six milk samples collected from 102 infected cows in different Tunisian regions were analysed. M. bovis isolates were further characterized by spoligotyping and variable number tandem repeat typing. Results: A total of five (4.9 %) M. bovis strains exhibiting three different genotypes were isolated. Conclusion: This study demonstrates that consumers of raw milk or derivatives in Tunisia are at high risk of zoonotic infection with M. bovis.
ISSN:1680-6905
1729-0503
1680-6905
DOI:10.4314/ahs.v11i3.70032