Bone matrix turnover and balance in vitro. II. The effects of aging

The rates of both formation and resorption of bone collagen may be accurately quantitated by kinetic analysis of hydroxyproline metabolism in vitro. Using this approach we have studied the changes in bone collagen turnover with age in the rat. The rates of synthesis and resorption of collagen declin...

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Bibliographic Details
Published in:The Journal of clinical investigation 1969-04, Vol.48 (4), p.607-612
Main Authors: Flanagan, B, Nichols, Jr, G
Format: Article
Language:English
Subjects:
Aging
Animals
Bone and Bones - metabolism
Bone Resorption
Collagen - biosynthesis
DNA - analysis
Hydroxyproline - metabolism
In Vitro Techniques
Male
Osteoporosis - etiology
Osteoporosis - physiopathology
Rats
Solubility
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Summary:The rates of both formation and resorption of bone collagen may be accurately quantitated by kinetic analysis of hydroxyproline metabolism in vitro. Using this approach we have studied the changes in bone collagen turnover with age in the rat. The rates of synthesis and resorption of collagen decline with age although the resorptive activity per cell increases up to 6 months of age. The solubility of collagen declines with age. The fraction of the newly synthesized collagen which is deposited as matrix declines dramatically with age revealing a new and hitherto unsuspected aspect of the osteoporotic process. The collagen balance becomes progressively more negative over the 1st 6 months of life. These results indicate that even in an animal who is not subject to clinical osteoporosis, biochemical measurement reveals that such a trend exists. The application of this approach to human subjects is feasible and has important implications.
ISSN:0021-9738
DOI:10.1172/JCI106019