Use of a centrifugal bioreactor for cartilaginous tissue formation from isolated chondrocytes

• Published in: Biotechnology progress 2011-03, Vol.27 (2), p.451-459
• Main Authors: Detzel, Christopher J., Van Wie, Bernard J.
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Bioreactors; Biotechnology; Buoyancy; cartilage; Cartilage - chemistry; Cell culture; Cell Culture Techniques - methods; Cell density; Cell Separation - methods; centrifugal bioreactor; Centrifugation; chondrocyte; Chondrocytes; Chondrocytes - cytology; Chondrogenesis; Collagen; Collagen - analysis; Continuous culture; DNA; Fundamental and applied biological sciences. Psychology; Glycosaminoglycans; Glycosaminoglycans - analysis; high density; Humans; hydrostatic pressure; Integration; Methods. Procedures. Technologies; Pressure; scaffolds; shear force; Stress; Tissue Engineering - methods; Various methods and equipments
• ISSN: 8756-7938; 1520-6033; 1520-6033
• DOI: 10.1002/btpr.551

Although a centrifugal bioreactor (CCBR) supports high‐density mammalian suspension cell cultures by balancing drag, buoyancy, and centrifugal forces, to date anchorage‐dependent cultures have not been tried. Also, steady or intermittent hydrostatic pressures of 8 to 500 kPa, and shears of 0.02 to 1.4 N/m2 can be simultaneously applied in the CCBR. This article demonstrates the use of a CCBR to stimulate chondrogenesis in a high‐density culture. At 3 weeks, histological results show even distribution of glycosaminoglycan (GAG) and collagen, with 1,890 ± 270 cells/mm2 cell densities that exceed those of 1,470 ± 270 in pellet cultures. Analysis of collagen content reveals similar levels for all treatment groups; 6.8 ± 3.5 and 5.0 ± 0.4 μg collagen/μg DNA for 0.07 and 0.26 MPa CCBR cultures, respectively, in contrast to 6.6 ± 1.9 values for control pellet cultures. GAG levels of 5.6 ± 1.5 and 4.1 ± 0.9 μg GAG /μg DNA are present for cultures stressed at 0.07 and 0.26 MPa, respectively, in comparison to control pellet cultures at the 8.4 ± 0.9 level. Although results to date have not revealed mechanical stress combinations that stimulate chondrogenesis over unstressed controls, system advantages include continuous culture at cell densities above those in the pellet, precise medium control, the ability to independently vary multiple mechanical stresses over a broad range, and the flexibility for integration of scaffold features for future chondrogenesis stimulation studies. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011