Maf1 Protein, Repressor of RNA Polymerase III, Indirectly Affects tRNA Processing

Maf1 is negative regulator of RNA polymerase III in yeast. We observed high levels of both primary transcript and end-matured, intron-containing pre-tRNAs in the maf1Δ strain. This pre-tRNA accumulation could be overcome by transcription inhibition, arguing against a direct role of Maf1 in tRNA matu...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 2011-11, Vol.286 (45), p.39478-39488
Main Authors: Karkusiewicz, Iwona, Turowski, Tomasz W., Graczyk, Damian, Towpik, Joanna, Dhungel, Nripesh, Hopper, Anita K., Boguta, Magdalena
Format: Article
Language:English
Subjects:
Active Transport, Cell Nucleus - physiology
Cell Nucleus - genetics
Cell Nucleus - metabolism
Gene Deletion
Maf1
Models, Biological
Nuclear Pore Complex Proteins - genetics
Nuclear Pore Complex Proteins - metabolism
RNA
RNA Polymerase III
RNA Polymerase III - genetics
RNA Polymerase III - metabolism
RNA Precursors - biosynthesis
RNA Precursors - genetics
RNA Processing, Post-Transcriptional - physiology
RNA Transport
RNA, Fungal - biosynthesis
RNA, Fungal - genetics
RNA, Transfer - biosynthesis
RNA, Transfer - genetics
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Transcription Factors - genetics
Transcription Factors - metabolism
Transcription Repressor
Transfer RNA (tRNA)
tRNA Nuclear Export
Yeast
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Maf1 is negative regulator of RNA polymerase III in yeast. We observed high levels of both primary transcript and end-matured, intron-containing pre-tRNAs in the maf1Δ strain. This pre-tRNA accumulation could be overcome by transcription inhibition, arguing against a direct role of Maf1 in tRNA maturation and suggesting saturation of processing machinery by the increased amounts of primary transcripts. Saturation of the tRNA exportin, Los1, is one reason why end-matured intron-containing pre-tRNAs accumulate in maf1Δ cells. However, it is likely possible that other components of the processing pathway are also limiting when tRNA transcription is increased. According to our model, Maf1-mediated transcription control and nuclear export by Los1 are two major stages of tRNA biosynthesis that are regulated by environmental conditions in a coordinated manner.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M111.253310