Ixora parviflora Protects against UVB-Induced Photoaging by Inhibiting the Expression of MMPs, MAP Kinases, and COX-2 and by Promoting Type I Procollagen Synthesis

Ixora parviflora with high polyphenol content exhibited antioxidant activity and reducing UVB-induced intracellular reactive oxygen species production. In this study, results of the photoaging screening experiments revealed that IPE at 1000 μg/mL reduced the activity of bacterial collagenase by 92.7...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2012-01, Vol.2012 (2012), p.1-11
Main Authors: Shih, I-Chen, Tsai, Shang-Yuan, Fan, Pei-Ching, Wen, Kuo-Ching, Chiang, Hsiu-Mei
Format: Article
Language:English
Subjects:
Acids
Aging
Antioxidants
Archives & records
Biochemistry
Collagen
Collagenase
Cyclooxygenase-2
Elastase
Exposure
Extracellular matrix
Extracellular signal-regulated kinase
Fibroblasts
Gelatin
Gelatinase B
Inflammation
Interstitial collagenase
Irradiation
Ixora
Kinases
Matrix metalloproteinase
Melanoma
Nitric oxide
Phosphorylation
Polyphenols
Procollagen
Proteins
Reactive oxygen species
Secretion
Skin cancer
Smad7 protein
Ultraviolet radiation
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Summary:Ixora parviflora with high polyphenol content exhibited antioxidant activity and reducing UVB-induced intracellular reactive oxygen species production. In this study, results of the photoaging screening experiments revealed that IPE at 1000 μg/mL reduced the activity of bacterial collagenase by 92.7 ± 4.2% and reduced the activity of elastase by 32.6 ± 1.4%. Therefore, we investigated the mechanisms by which IPE exerts its anti-photoaging activity. IPE at 1 μg/mL led to an increase in type I procollagen expression and increased total collagen synthesis in fibroblasts at 5 μg/mL. We found that IPE inhibited MMP-1, MMP-3, and MMP-9 expression at doses of 1, 5, and 10 μg/mL, respectively, in fibroblasts exposed to UV irradiation (40 mJ/cm2). Gelatin zymography assay showed that IPE at 50 μg/mL inhibited MMP-9 secretion/activity in cultured fibroblasts after UVB exposure. In addition, IPE inhibited the phosphorylation of p38, ERK, and JNK induced by UVB. Furthermore, IPE inhibited the UVB-induced expression of Smad7. In addition, IPE at 1 μg/mL inhibited NO production and COX-2 expression in UV-exposed fibroblasts. These findings show that IPE exhibits anti-inflammatory and anti-photoaging activities, indicating that IPE could be a potential anti-aging agent.
ISSN:1741-427X
1741-4288
1741-4288
DOI:10.1155/2012/417346