Effects of DDA, CpG-ODN, and plasmid-encoded chicken IFN-γ on protective immunity by a DNA vaccine against IBDV in chickens

• Published in: Journal of veterinary science (Suwŏn-si, Korea) Korea), 2006-12, Vol.7 (4), p.361-368
• Main Authors: Roh, H.J. (Kangwon National University, Chuncheon, Republic of Korea), Sung, H.W. (Kangwon National University, Chuncheon, Republic of Korea), Kwon, H.M. (Kangwon National University, Chuncheon, Republic of Korea), E-mail: [e-mail]
• Format: Article
• Language: English
• Subjects: Adjuvants, Immunologic; Animals; Antibodies, Viral - blood; antibody formation; Birnaviridae Infections - immunology; Birnaviridae Infections - prevention & control; Birnaviridae Infections - virology; Bursa of Fabricius - immunology; Bursa of Fabricius - virology; Cell Proliferation; chicken IFN-gamma; Chickens; CpG Islands - immunology; CpG-ODN; cytosine-phosphate-guanosine oligodeoxynucleotides; DDA; dimethyl dioctadecyl ammonium bromide; DNA vaccine; Enzyme-Linked Immunosorbent Assay - veterinary; immune response; Immunization - methods; Immunization - veterinary; INFECTIOUS BURSAL DISEASE VIRUS; Infectious bursal disease virus - immunology; Interferon-gamma - immunology; Interferon-gamma - therapeutic use; interferons; intramuscular injection; intraperitoneal injection; lymphocyte proliferation; Lymphocytes - cytology; Lymphocytes - immunology; mortality; Oligonucleotides - immunology; Original; Poultry Diseases - immunology; Poultry Diseases - prevention & control; Poultry Diseases - virology; Specific Pathogen-Free Organisms; vaccination; vaccine adjuvants; vaccine development; vaccines; Vaccines, DNA - immunology; Vaccines, DNA - therapeutic use; Viral Vaccines - immunology; Viral Vaccines - therapeutic use; VIRUS BURSITE INFECTIEUSE; VIRUS BURSITIS INFECCIOSA
• ISSN: 1229-845X; 1976-555X
• DOI: 10.4142/jvs.2006.7.4.361

This study examined the adjuvant effects of dimethyl dioctadecyl ammonium bromide (DDA), CpG oligodeoxynucleotides (CpG-ODN), and chicken interferon-γ (ChIFN-γ) on a DNA vaccine (pcDNA-VP243) against the infectious bursal disease virus (IBDV). A plasmid encoding chicken IFN-a was constructed. Twice at 2-week intervals, two-week-old chickens were injected intramuscularly and intraperitoneally with either a DNA vaccine alone or a DNA vaccine together with the respective adjuvants. On week 2 after the second immunization, the chickens were orally challenged with the highly virulent IBDV.