A mammalian mitochondrial serine transfer RNA lacking the “dihydrouridine” loop and stem

A unique transfer DNA has been identified in human and bovine mitochondria that lacks the “dihydrouridine” loop and stem structure. This tRNA ie mitochondrially coded as shown by DNA sequence analysis of the human and bovine mitochondrial DNA. Sequence analysis of the INA shows that it is post-trans...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 1980-11, Vol.8 (22), p.5213-5222
Main Authors: de Bruijn, M.H.L., Schreier, P.H., Eperon, I.C., Barrell, B.G., Chen, E.Y., Armstrong, P.W., Wong, J.F.H., Roe, B.A.
Format: Article
Language:English
Subjects:
Animals
Anticodon
Base Sequence
Cattle
Cloning, Molecular
DNA, Recombinant - metabolism
Humans
Mitochondria, Liver - metabolism
Nucleic Acid Conformation
Plasmids
RNA, Transfer, Amino Acyl - analysis
RNA, Transfer, Amino Acyl - biosynthesis
Species Specificity
Uridine - analogs & derivatives
Uridine - analysis
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A unique transfer DNA has been identified in human and bovine mitochondria that lacks the “dihydrouridine” loop and stem structure. This tRNA ie mitochondrially coded as shown by DNA sequence analysis of the human and bovine mitochondrial DNA. Sequence analysis of the INA shows that it is post-transcriptionally modified by the addition of CCA at the 3′ terminus and that at least one base is modified. As predicted by its anticodon (GCU, corresponding to the serine codons AGU/C) this tRNA can be aminoacylated with serine when purified mitochondria are incubated in a medium containing 3H-serine.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/8.22.5213