A new restriction endonuclease from the anaerobic bacterium, Desulfovibrio desulfuricans, Norway

The purification and characterization of a new restriction endonu-clease, Dde 1, from a sulfate-reducing, anaerobic bacterium, Desulfovibrio desulfuricans, Norway, is reported. The enzyme recognizes the sequence 5′-C -↓T-N-A-G-3′3′-G-A-N-T-↑C-5′ and cleaves at the position indicated by the arrows. T...

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Bibliographic Details
Published in:Nucleic acids research 1980-07, Vol.8 (14), p.3125-3132
Main Authors: Makula, Ronald A., Meagher, Richard B.
Format: Article
Language:English
Subjects:
Bacteria
Base Sequence
Deoxyribonucleases, Type II Site-Specific
Desulfovibrio - enzymology
Desulfovibrio desulfuricans
DNA Restriction Enzymes - isolation & purification
DNA Restriction Enzymes - metabolism
DNA, Viral - metabolism
Electrophoresis, Polyacrylamide Gel
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Summary:The purification and characterization of a new restriction endonu-clease, Dde 1, from a sulfate-reducing, anaerobic bacterium, Desulfovibrio desulfuricans, Norway, is reported. The enzyme recognizes the sequence 5′-C -↓T-N-A-G-3′3′-G-A-N-T-↑C-5′ and cleaves at the position indicated by the arrows. The enzyme preparation obtained is suitable for restriction mapping and ligation.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/8.14.3125