Characterization of cloned ribosomal DNA from Drosophila hydei

The structure of ribosomal genes from the fly Dŕosophila hydei has been analyzed. EcoRI fragments, cloned in a plasmid vector, were mapped by restriction enzyme analysis. The lengths of the regions coding for 18S and 28S rRNA were defined by R-loop formation. From these data a physical map of the rR...

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Bibliographic Details
Published in:Nucleic acids research 1980-10, Vol.8 (20), p.4593-4611
Main Authors: Renkawitz-Pohl, R, Glatzer, K.H, Kunz, W
Format: Article
Language:English
Subjects:
animal breeding
animal genetics
Animals
arthropods
Cloning, Molecular
DNA Restriction Enzymes
DNA, Recombinant - metabolism
Drosophila - metabolism
Drosophila hydei
entomology
Genes
Microscopy, Electron
Molecular Weight
Nucleic Acid Conformation
Plasmids
RNA, Ribosomal - biosynthesis
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Summary:The structure of ribosomal genes from the fly Dŕosophila hydei has been analyzed. EcoRI fragments, cloned in a plasmid vector, were mapped by restriction enzyme analysis. The lengths of the regions coding for 18S and 28S rRNA were defined by R-loop formation. From these data a physical map of the rRNA genes was constructed. There are two major types of rDNA units in D. hydei one having a size of 11 kb and the other a size of 17 kb. The 17 kb unit results from an intervening sequence (ivs) of 6.0 kb, interrupting the β-28S rRNA coding region. Some homology between the D. hydei ivs and D. melanogaster type 1 ivs has been described previously (1). However, the restriction sites within these ivs show considerable divergence. Whereas D. hydei rDNA sequences coding for mature rRNAs share restriction sites with D. melanogaster rDNA, the nontranscribed spacer has little, if any, sequence homology. Despite difference in sequence, D. hydei and D. melanogaster spacers show structural similarities in that both contain repeated sequence elements of similar size and location.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/8.20.4593