The C. elegans microRNA let-7 binds to imperfect let-7 complementary sites from the lin-41 3′UTR

Caenorhabditis elegans let-7 , a founding member of the microRNA family, is predicted to bind to six sites in the 3′UTR of the mRNA of its target gene, lin-41 , to down-regulate LIN-41. Here, we demonstrate that wild-type let-7 microRNA binds in vitro to RNA from the lin-41 3′UTR. This interaction i...

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Bibliographic Details
Published in:Genes & development 2004-01, Vol.18 (2), p.132-137
Main Authors: Vella, Monica C., Choi, Eun-Young, Lin, Shin-Yi, Reinert, Kristy, Slack, Frank J.
Format: Article
Language:English
Subjects:
Research Communications
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Summary:Caenorhabditis elegans let-7 , a founding member of the microRNA family, is predicted to bind to six sites in the 3′UTR of the mRNA of its target gene, lin-41 , to down-regulate LIN-41. Here, we demonstrate that wild-type let-7 microRNA binds in vitro to RNA from the lin-41 3′UTR. This interaction is dependent on two conserved let-7 complementary sites (LCSs). A 27-nucleotide sequence between the LCSs is also necessary for down-regulation in vivo. LCS mutations compensatory to the lesion in let-7(n2853) can partially restore lin-41 3′UTR function in a let-7(n2853) background, providing the first experimental evidence for an animal miRNA binding directly to its validated target in vivo.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.1165404