A methylation-mediator complex in hormone signaling

The recruitment of coactivators by nuclear hormone receptors (NRs) promotes transcription by subverting chromatin-mediated repression. Although the histone methylation enzyme CARM1 and an ATP-remodeling complex have been individually implicated in nuclear receptor-dependent transcription, neither a...

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Bibliographic Details
Published in:Genes & development 2004-01, Vol.18 (2), p.144-156
Main Authors: Xu, Wei, Cho, Helen, Kadam, Shilpa, Banayo, Ester M, Anderson, Scott, Yates, 3rd, John R, Emerson, Beverly M, Evans, Ronald M
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Animals
CARM1 protein
Chromosomal Proteins, Non-Histone - metabolism
coactivators
Humans
Macromolecular Substances
Methylation
Methyltransferases - metabolism
Protein-Arginine N-Methyltransferases - isolation & purification
Protein-Arginine N-Methyltransferases - metabolism
Receptors, Cytoplasmic and Nuclear - metabolism
Research Papers
Signal Transduction - physiology
Substrate Specificity
SWI/SNF complex
Transcription Factors - metabolism
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Summary:The recruitment of coactivators by nuclear hormone receptors (NRs) promotes transcription by subverting chromatin-mediated repression. Although the histone methylation enzyme CARM1 and an ATP-remodeling complex have been individually implicated in nuclear receptor-dependent transcription, neither a functional nor mechanistic linkage between these systems has been identified. In the process of purifying endogenous CARM1-interacting proteins, we identified an associated complex, nucleosomal methylation activator complex (NUMAC), which includes at least eight components of SWI/SNF, including the ATPase BRG1. In the NUMAC complex, the methylase, CARM1, acquires the ability to covalently modify nucleosomal histones, and the directed nucleosome versus free core histone methylation-specificity change is increased dramatically. Reciprocally, CARM1 stimulates the ATPase activity of BRG1, a key component in nucleosome remodeling. In vivo, CARM1 and BRG1 coassemble on an estrogen receptor (ER)-target gene to cooperatively activate ER-dependent transcription. This association of ATP-remodeling factors with HMT CARM1 defines a new component of regulation in the nuclear hormone-signaling pathway.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.1141704