Cysteamine suppresses human peripheral blood mononuclear cells--human corneal endothelial cell reaction via reactive oxygen species reduction

To investigate the effect of cysteamine (CYS) on mixed peripheral blood mononuclear cells (PBMCs)--human corneal endothelial cell (HCEC) reaction (MLER). PBMC stimulation assay was performed using cultured HCEC. MLERs were treated with various concentrations of CYS (0-20 mM). The proliferation rate...

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Bibliographic Details
Published in:Molecular vision 2011-12, Vol.17, p.3371-3378
Main Authors: Shin, Young Joo, Hyon, Joon Young, Kim, Seonhowa, Koh, Jae Woong, Kwon, Soon Il, Wee, Won Ryang
Format: Article
Language:English
Subjects:
Cell Proliferation - drug effects
Cells, Cultured
Coculture Techniques
Cornea - cytology
Cornea - drug effects
Cornea - metabolism
Cysteamine - pharmacology
Dose-Response Relationship, Drug
Endothelial Cells - cytology
Endothelial Cells - drug effects
Endothelial Cells - metabolism
Enzyme-Linked Immunosorbent Assay
Fluorescent Dyes
Humans
Inflammation - prevention & control
Interleukin-6 - biosynthesis
Leukocytes, Mononuclear - cytology
Leukocytes, Mononuclear - drug effects
Leukocytes, Mononuclear - metabolism
Radiation-Protective Agents - pharmacology
Reactive Oxygen Species - antagonists & inhibitors
Reactive Oxygen Species - metabolism
Spectrometry, Fluorescence
Transforming Growth Factor beta1 - biosynthesis
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Summary:To investigate the effect of cysteamine (CYS) on mixed peripheral blood mononuclear cells (PBMCs)--human corneal endothelial cell (HCEC) reaction (MLER). PBMC stimulation assay was performed using cultured HCEC. MLERs were treated with various concentrations of CYS (0-20 mM). The proliferation rate and secretion profiles of transforming growth factor-β1 (TGF-β1) and interleukin-6 (IL-6) of PBMCs stimulated by cultured HCEC were determined using bromodeoxyuridine proliferation assay and enzyme-linked immunosorbent assay, respectively. CYS suppressed PBMC proliferation in a dose-dependent manner (p
ISSN:1090-0535
1090-0535