Evaluation of Septa Quality for Automatic SPME-GC-MS Trace Analysis

The vials used for the preparation of breath samples for automated solid-phase microextraction-gas chromatography-mass spectrometry analysis are crimped with septa. These septa often emit specific volatile organic compounds (VOCs) confounding the measurement results of breath samples. In the current...

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Bibliographic Details
Published in:Journal of chromatographic science 2012-01, Vol.50 (1), p.10-14
Main Authors: Ulanowska, Agnieszka, Ligor, Tomasz, Amann, Anton, Buszewski, Bogusław
Format: Article
Language:English
Subjects:
Acetone - analysis
Acetone - chemistry
Analytical chemistry
Breath Tests - instrumentation
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Gas chromatographic methods
Gas Chromatography-Mass Spectrometry - instrumentation
Humans
Polytetrafluoroethylene - chemistry
Reproducibility of Results
Rubber - chemistry
Silicones - chemistry
Solid Phase Microextraction - instrumentation
Toluene - analysis
Toluene - chemistry
Volatile Organic Compounds - analysis
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Summary:The vials used for the preparation of breath samples for automated solid-phase microextraction-gas chromatography-mass spectrometry analysis are crimped with septa. These septa often emit specific volatile organic compounds (VOCs) confounding the measurement results of breath samples. In the current paper, 14 different brands of magnetic caps with silicone-polytetrafluoroethylene (PTFE), butyl-PTFE, or butyl rubber septa were tested. The total emission of septa over a 4 h period was also evaluated. The tested septa emitted 39 different compounds, which are mainly hydrocarbons, alcohols, and ketones. Acetone and toluene are the most abundant out-gassing products. The concentration of acetone was in the range from 55 to 694 ppb for butyl-PTFE septum (brand 14) and butyl rubber (brand 10), respectively. The measured toluene amount was 69-1323 ppb for the septum brand 14 and brand 8 (silicone-PTFE), respectively. Generally, the butyl rubber septa released higher amounts of contaminants in comparison to the silicone ones.
ISSN:0021-9665
1945-239X
DOI:10.1093/chromsci/bmr006