Dopamine release at individual presynaptic terminals visualized with FFNs

The nervous system transmits signals between neurons via neurotransmitter release during synaptic vesicle fusion. To observe neurotransmitter uptake and release from individual presynaptic terminals directly, we designed fluorescent false neurotransmitters as substrates for the synaptic vesicle mono...

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Bibliographic Details
Published in:Journal of visualized experiments 2009-08 (30)
Main Authors: Zhang, Hui, Gubernator, Niko G, Yue, Minerva, Staal, Roland G W, Mosharov, Eugene V, Pereira, Daniela, Balsanek, Vojtech, Vadola, Paul A, Mukherjee, Bipasha, Edwards, Robert H, Sulzer, David, Sames, Dalibor
Format: Article
Language:English
Subjects:
Animals
Benz(a)Anthracenes - analysis
Corpus Striatum - chemistry
Corpus Striatum - metabolism
Dopamine - analysis
Dopamine - metabolism
Fluorescent Dyes - analysis
Male
Mice
Neuronal Plasticity
Neuroscience
Presynaptic Terminals - chemistry
Presynaptic Terminals - metabolism
Receptors, Dopamine D2 - metabolism
Synaptic Transmission
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Summary:The nervous system transmits signals between neurons via neurotransmitter release during synaptic vesicle fusion. To observe neurotransmitter uptake and release from individual presynaptic terminals directly, we designed fluorescent false neurotransmitters as substrates for the synaptic vesicle monoamine transporter. Using these probes to image dopamine release in the striatum, we made several observations pertinent to synaptic plasticity. We found that the fraction of synaptic vesicles releasing neurotransmitter per stimulus was dependent on the stimulus frequency. A kinetically distinct "reserve" synaptic vesicle population was not observed under these experimental conditions. A frequency-dependent heterogeneity of presynaptic terminals was revealed that was dependent in part on D2 dopamine receptors, indicating a mechanism for frequency-dependent coding of presynaptic selection.
ISSN:1940-087X
1940-087X
DOI:10.3791/1562