Time-resolved imaging refractometry of microbicidal films using quantitative phase microscopy

Quantitative phase microscopy is applied to image temporal changes in the refractive index (RI) distributions of solutions created by microbicidal films undergoing hydration. We present a novel method of using an engineered polydimethylsiloxane structure as a static phase reference to facilitate cal...

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Bibliographic Details
Published in:Journal of Biomedical Optics 2011-12, Vol.16 (12), p.120510-120510
Main Authors: Rinehart, Matthew T, Drake, Tyler K, Robles, Francisco E, Wax, Adam P, Rohan, Lisa C, Katz, David F
Format: Article
Language:English
Subjects:
Anti-Infective Agents - chemistry
Dimethylpolysiloxanes - chemistry
JBO Letters
Microscopy - methods
Pharmaceutical Vehicles - chemistry
Refractometry - methods
Water - chemistry
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Summary:Quantitative phase microscopy is applied to image temporal changes in the refractive index (RI) distributions of solutions created by microbicidal films undergoing hydration. We present a novel method of using an engineered polydimethylsiloxane structure as a static phase reference to facilitate calibration of the absolute RI across the entire field. We present a study of dynamic structural changes in microbicidal films during hydration and subsequent dissolution. With assumptions about the smoothness of the phase changes induced by these films, we calculate absolute changes in the percentage of film in regions across the field of view.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.3665439