NMR-metabolic methodology in the study of GM foods

The 1H-NMR methodology used in the study of genetically modified (GM) foods is discussed. Transgenic lettuce (Lactuca sativa cv "Luxor") over-expressing the ArabidopsisKNAT1 gene is presented as a case study. Twenty-two water-soluble metabolites (amino acids, organic acids, sugars) present...

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Bibliographic Details
Published in:Nutrients 2010-01, Vol.2 (1), p.1-15
Main Authors: Sobolev, Anatoly P, Capitani, Donatella, Giannino, Donato, Nicolodi, Chiara, Testone, Giulio, Santoro, Flavio, Frugis, Giovanna, Iannelli, Maria A, Mattoo, Autar K, Brosio, Elvino, Gianferri, Raffaella, D'Amico, Irene, Mannina, Luisa
Format: Article
Language:English
Subjects:
Amino Acids - metabolism
Arabidopsis - genetics
Arabidopsis Proteins - metabolism
Carbohydrate Metabolism
Carboxylic Acids - metabolism
Homeodomain Proteins - metabolism
Humans
Lactuca - chemistry
Lactuca - genetics
Magnetic Resonance Spectroscopy
Plant Leaves - chemistry
Plant Leaves - genetics
Plants, Genetically Modified - chemistry
Plants, Genetically Modified - genetics
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Summary:The 1H-NMR methodology used in the study of genetically modified (GM) foods is discussed. Transgenic lettuce (Lactuca sativa cv "Luxor") over-expressing the ArabidopsisKNAT1 gene is presented as a case study. Twenty-two water-soluble metabolites (amino acids, organic acids, sugars) present in leaves of conventional and GM lettuce were monitored by NMR and quantified at two developmental stages. The NMR spectra did not reveal any difference in metabolite composition between the GM lettuce and the wild type counterpart. Statistical analyses of metabolite variables highlighted metabolism variation as a function of leaf development as well as the transgene. A main effect of the transgene was in altering sugar metabolism.
ISSN:2072-6643
DOI:10.3390/nu20100001