NMR-metabolic methodology in the study of GM foods

• Published in: Nutrients 2010-01, Vol.2 (1), p.1-15
• Main Authors: Sobolev, Anatoly P, Capitani, Donatella, Giannino, Donato, Nicolodi, Chiara, Testone, Giulio, Santoro, Flavio, Frugis, Giovanna, Iannelli, Maria A, Mattoo, Autar K, Brosio, Elvino, Gianferri, Raffaella, D'Amico, Irene, Mannina, Luisa
• Format: Article
• Language: English
• Subjects: Amino Acids - metabolism; Arabidopsis - genetics; Arabidopsis Proteins - metabolism; Carbohydrate Metabolism; Carboxylic Acids - metabolism; Homeodomain Proteins - metabolism; Humans; Lactuca - chemistry; Lactuca - genetics; Magnetic Resonance Spectroscopy; Plant Leaves - chemistry; Plant Leaves - genetics; Plants, Genetically Modified - chemistry; Plants, Genetically Modified - genetics
• ISSN: 2072-6643
• DOI: 10.3390/nu20100001

The 1H-NMR methodology used in the study of genetically modified (GM) foods is discussed. Transgenic lettuce (Lactuca sativa cv "Luxor") over-expressing the ArabidopsisKNAT1 gene is presented as a case study. Twenty-two water-soluble metabolites (amino acids, organic acids, sugars) present in leaves of conventional and GM lettuce were monitored by NMR and quantified at two developmental stages. The NMR spectra did not reveal any difference in metabolite composition between the GM lettuce and the wild type counterpart. Statistical analyses of metabolite variables highlighted metabolism variation as a function of leaf development as well as the transgene. A main effect of the transgene was in altering sugar metabolism.