Proteomic Analysis of Trypanosoma cruzi Epimastigotes Subjected to Heat Shock

Trypanosoma cruzi is exposed to sudden temperature changes during its life cycle. Adaptation to these variations is crucial for parasite survival, reproduction, and transmission. Some of these conditions may change the pattern of genetic expression of proteins involved in homeostasis in the course o...

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Bibliographic Details
Published in:Journal of biomedicine & biotechnology 2012-01, Vol.2012 (2012), p.1-9
Main Authors: Pérez-Morales, Deyanira, Lanz-Mendoza, Humberto, Hurtado, Gerardo, Martínez-Espinosa, Rodrigo, Espinoza, Bertha
Format: Article
Language:English
Subjects:
Adaptation, Physiological
Electrophoresis, Gel, Two-Dimensional - methods
Heat-Shock Response - physiology
Humans
Mass Spectrometry - methods
Proteins - analysis
Proteins - classification
Proteins - metabolism
Proteome - analysis
Proteome - metabolism
Trypanosoma cruzi - metabolism
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Summary:Trypanosoma cruzi is exposed to sudden temperature changes during its life cycle. Adaptation to these variations is crucial for parasite survival, reproduction, and transmission. Some of these conditions may change the pattern of genetic expression of proteins involved in homeostasis in the course of stress treatment. In the present study, the proteome of T. cruzi epimastigotes subjected to heat shock and epimastigotes grow normally was compared by two-dimensional gel electrophoresis followed by mass spectrometry for protein identification. Twenty-four spots differing in abundance were identified. Of the twenty-four changed spots, nineteen showed a greater intensity and five a lower intensity relative to the control. Several functional categories of the identified proteins were determined: metabolism, cell defense, hypothetical proteins, protein fate, protein synthesis, cellular transport, and cell cycle. Proteins involved in the interaction with the cellular environment were also identified, and the implications of these changes are discussed.
ISSN:1110-7243
1110-7251
DOI:10.1155/2012/902803