Nucleotide sequence of the 5′ noncoding region and part of the gag gene of mouse mammary tumor virus; identification of the 5′ splicing site for subgenomic mRNAs

We have determined the sequence of the first 1371 nucleotides at the 5′; end of the genome of mouse mammary tumor virus using molecularly cloned proviral DNA of the GR virus strain. The most likely initiation codon used for the gag gene of mouse mammary tumor virus is the first one, located 312 nucl...

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Bibliographic Details
Published in:Nucleic acids research 1983-10, Vol.11 (20), p.6943-6955
Main Authors: Fasel, N., Buetti, E., Firzlaff, J., Pearson, K., Diggelmann, H.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antigens, Viral - genetics
Base Sequence
Biological and medical sciences
Cloning, Molecular
Codon - genetics
Coliphages - genetics
DNA - analysis
DNA Restriction Enzymes
Fundamental and applied biological sciences. Psychology
Gene Products, gag
Genes
Genes, Viral
Genes. Genome
Mammary Tumor Virus, Mouse - genetics
Mammary Tumor Virus, Mouse - immunology
Molecular and cellular biology
Molecular genetics
Mouse mammary tumor virus
RNA, Messenger - genetics
Viral Proteins - genetics
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Summary:We have determined the sequence of the first 1371 nucleotides at the 5′; end of the genome of mouse mammary tumor virus using molecularly cloned proviral DNA of the GR virus strain. The most likely initiation codon used for the gag gene of mouse mammary tumor virus is the first one, located 312 nucleotides from the 5′ end of the viral RNA. The 5′ splicing site for the subgenomic mRNA's is located ∼ 288 nucleotides downstream from the 5′ end of the viral RNA. From the DNA sequence the amino acid sequence of the N-terminal half of the gag precursor protein, including p10 and p21, was deduced (353 amino acids).
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/11.20.6943