Rhythmic actomyosin-driven contractions induced by sperm entry predict mammalian embryo viability

• Published in: Nature communications 2011-08, Vol.2 (1), p.417-417, Article 417
• Main Authors: Ajduk, Anna, Ilozue, Tagbo, Windsor, Shane, Yu, Yuansong, Seres, K. Bianka, Bomphrey, Richard J., Tom, Brian D., Swann, Karl, Thomas, Adrian, Graham, Chris, Zernicka-Goetz, Magdalena
• Format: Article
• Language: English
• Subjects: 631/136/2086; 631/443/494; 631/80/128; Actomyosin - metabolism; Animals; Cell Survival; Cytoplasmic Streaming; Embryo, Mammalian - metabolism; Female; Fertilization; Fertilization in Vitro; Humanities and Social Sciences; Male; Mice; Mice, Inbred C57BL; Mice, Inbred CBA; multidisciplinary; Ovum - metabolism; Science; Science (multidisciplinary); Sperm-Ovum Interactions; Spermatozoa - metabolism; Zygote - metabolism
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/ncomms1424

Fertilization-induced cytoplasmic flows are a conserved feature of eggs in many species. However, until now the importance of cytoplasmic flows for the development of mammalian embryos has been unknown. Here, by combining a rapid imaging of the freshly fertilized mouse egg with advanced image analysis based on particle image velocimetry, we show that fertilization induces rhythmical cytoplasmic movements that coincide with pulsations of the protrusion forming above the sperm head. We find that these movements are caused by contractions of the actomyosin cytoskeleton triggered by Ca 2+ oscillations induced by fertilization. Most importantly, the relationship between the movements and the events of egg activation makes it possible to use the movements alone to predict developmental potential of the zygote. In conclusion, this method offers, thus far, the earliest and fastest, non-invasive way to predict the viability of eggs fertilized in vitro and therefore can potentially improve greatly the prospects for IVF treatment. Cytoplasmic flows—the movement of cytoplasmic material—can be detected following the fertilization of an egg by a sperm in many species. In this study, rhythmic cytoplasmic flows are shown to be induced in mice by calcium-induced cytoskeleton contractions which could be used to predict the successful outcome of fertilization.