Synthesis of short RNA fragments by the benzotriazolyl phosphotriester approach

Analytical and experimental evidence will be presented to show that addition of a slight excess of 2-chlorophenyl-0,0-bis[1-benzotriazolyl]phosphate to a properly 2′,5′-protected ribonucleoside gives an intermediate 3′-phosphotriester, which, after addition of a 2′-protected ribonucleoside, affords...

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Bibliographic Details
Published in:Nucleic acids research 1983-12, Vol.11 (23), p.8389-8405
Main Authors: Wreesmann, C.T.J., Fidder, A., van der Marel, G.A., van Boom, J.H.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Base Sequence
Biological and medical sciences
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Fundamental and applied biological sciences. Psychology
Indicators and Reagents
Nucleic acids
Oligonucleotides - chemical synthesis
oligoribonucleotides
Oligoribonucleotides - chemical synthesis
phosphotriester method
RNA - chemical synthesis
Rna, ribonucleoproteins
Structure-Activity Relationship
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Summary:Analytical and experimental evidence will be presented to show that addition of a slight excess of 2-chlorophenyl-0,0-bis[1-benzotriazolyl]phosphate to a properly 2′,5′-protected ribonucleoside gives an intermediate 3′-phosphotriester, which, after addition of a 2′-protected ribonucleoside, affords a dimer containing solely a 3′-5′-internucleotide linkage. The above phosphorylation procedure has been used in the synthesis of the pentamer UpApCpGpC.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/11.23.8389