Interaction of diamidino-2-phenylindole (DAPI) with natural and synthetic nucleic acids

The interaction of DAPI with natural and synthetic polydeoxynucleotides of different base content and sequences was studied with circular dichroism, ultracentrifugation, viscosity and calorimetry. All the polymers show two types of binding. The strength of the interaction and its resistence to ionic...

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Bibliographic Details
Published in:Nucleic acids research 1983-12, Vol.11 (24), p.8861-8876
Main Authors: Manzini, G., Barcellona, M.L., Avitabile, M., Quadrifoglio, F.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cattle
Chemical Phenomena
Chemistry
Circular Dichroism
DNA
DNA, Bacterial
DNA, Viral
Fluorescent Dyes
Fundamental and applied biological sciences. Psychology
Genes. Genome
Indoles
Kinetics
Molecular and cellular biology
Molecular genetics
Nucleic Acid Conformation
Osmolar Concentration
Polydeoxyribonucleotides
Structure-Activity Relationship
Thymus Gland
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Summary:The interaction of DAPI with natural and synthetic polydeoxynucleotides of different base content and sequences was studied with circular dichroism, ultracentrifugation, viscosity and calorimetry. All the polymers show two types of binding. The strength of the interaction and its resistence to ionic strength are related to the content of AT clusters in the chain. On the other hand, sedimentation measurements rule out an intercalation mechanism. A model of DAPI interaction with DNA, similar to that displayed by distamycin and netropsin, is proposed.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/11.24.8861