structure of the yeast ribosomal RNA genes. 3. Precise mapping of the 18 S and 25 S rRNA genes and structure of the adjacent regions

The 5′-termini of Saccharomyces cerevisiae 18 S and 25 S rRNA are precisely mapped within the sequence of the rDNA repeating unit. The 3′-termini of 25 S rRNA and 37 S pre-rRNA are located within a 548 bp segment of the rDNA repeating unit by the use of a DNA polymerase I extension technique. The an...

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Bibliographic Details
Published in:Nucleic acids research 1981-02, Vol.9 (4), p.789-799
Main Authors: Bayev, A.A, Georgiev, O.I, Hadjiolov, A.A, Skryabin, K.G, Zakharyev, V.M
Format: Article
Language:English
Subjects:
Base Sequence
Genes
Nucleic Acid Conformation
Nucleic Acid Hybridization
RNA, Ribosomal - analysis
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
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Summary:The 5′-termini of Saccharomyces cerevisiae 18 S and 25 S rRNA are precisely mapped within the sequence of the rDNA repeating unit. The 3′-termini of 25 S rRNA and 37 S pre-rRNA are located within a 548 bp segment of the rDNA repeating unit by the use of a DNA polymerase I extension technique. The analysis of the rDNA sequences at the structural gene boundaries reveals the presence of oligonucleotide repeats which may be involved in transcription or processing control mechanisms. The sequence of rDNA in the transcription termination region is determined and possible mechanisms shaping the 3′-end of 25 S rRNA are discussed.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/9.4.789