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Phases and domains in sphingomyelin–cholesterol membranes: structure and properties using EPR spin-labeling methods
EPR spin-labeling methods were used to investigate the order and fluidity of alkyl chains, the hydrophobicity of the membrane interior, and the order and motion of cholesterol molecules in coexisting phases and domains, or in a single phase of fluid-phase cholesterol/egg-sphingomyelin (Chol/ESM) mem...
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| Published in: | European biophysics journal 2012-02, Vol.41 (2), p.147-159 |
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| Language: | English |
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| container_title | European biophysics journal |
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| creator | Mainali, Laxman Raguz, Marija Subczynski, Witold K. |
| description | EPR spin-labeling methods were used to investigate the order and fluidity of alkyl chains, the hydrophobicity of the membrane interior, and the order and motion of cholesterol molecules in coexisting phases and domains, or in a single phase of fluid-phase cholesterol/egg-sphingomyelin (Chol/ESM) membranes with a Chol/ESM mixing ratio from 0 to 3. A complete set of profiles for these properties was obtained for the liquid-disordered (
l
d
) phase without cholesterol, for the liquid-ordered (
l
o
) phase for the entire region of cholesterol solubility in this phase (from 33 to 66 mol%), and for the
l
o
-phase domain that coexists with the cholesterol bilayer domain (CBD). Alkyl chains in the
l
o
phase are more ordered than in the
l
d
pure ESM membrane. However, fluidity in the membrane center is greater. Also, the profile of hydrophobicity changed from a bell to a rectangular shape. These differences are enhanced when the cholesterol content of the
l
o
phase is increased from 33 to 66 mol%, with clear brake-points between the C9 and C10 positions (approximately where the steroid-ring structure of cholesterol reaches into the membrane). The organization and motion of cholesterol molecules in the CBD are similar to those in the
l
o
-phase domain that coexists with the CBD. |
| doi_str_mv | 10.1007/s00249-011-0766-4 |
| format | article |
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l
d
) phase without cholesterol, for the liquid-ordered (
l
o
) phase for the entire region of cholesterol solubility in this phase (from 33 to 66 mol%), and for the
l
o
-phase domain that coexists with the cholesterol bilayer domain (CBD). Alkyl chains in the
l
o
phase are more ordered than in the
l
d
pure ESM membrane. However, fluidity in the membrane center is greater. Also, the profile of hydrophobicity changed from a bell to a rectangular shape. These differences are enhanced when the cholesterol content of the
l
o
phase is increased from 33 to 66 mol%, with clear brake-points between the C9 and C10 positions (approximately where the steroid-ring structure of cholesterol reaches into the membrane). The organization and motion of cholesterol molecules in the CBD are similar to those in the
l
o
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l
d
) phase without cholesterol, for the liquid-ordered (
l
o
) phase for the entire region of cholesterol solubility in this phase (from 33 to 66 mol%), and for the
l
o
-phase domain that coexists with the cholesterol bilayer domain (CBD). Alkyl chains in the
l
o
phase are more ordered than in the
l
d
pure ESM membrane. However, fluidity in the membrane center is greater. Also, the profile of hydrophobicity changed from a bell to a rectangular shape. These differences are enhanced when the cholesterol content of the
l
o
phase is increased from 33 to 66 mol%, with clear brake-points between the C9 and C10 positions (approximately where the steroid-ring structure of cholesterol reaches into the membrane). The organization and motion of cholesterol molecules in the CBD are similar to those in the
l
o
-phase domain that coexists with the CBD.</description><subject>Biochemistry</subject><subject>Biological and Medical Physics</subject><subject>Biomedical and Life Sciences</subject><subject>Biophysics</subject><subject>Cell Biology</subject><subject>Cell Membrane - chemistry</subject><subject>Cholesterol</subject><subject>Cholesterol - chemistry</subject><subject>Electron Spin Resonance Spectroscopy</subject><subject>Hydrophobic and Hydrophilic Interactions</subject><subject>Life Sciences</subject><subject>Lipids</subject><subject>Membrane Biology</subject><subject>Membrane Fluidity</subject><subject>Membranes</subject><subject>Nanotechnology</subject><subject>Neurobiology</subject><subject>Original Paper</subject><subject>Scientific method</subject><subject>Sphingomyelins - chemistry</subject><subject>Spin Labels</subject><issn>0175-7571</issn><issn>1432-1017</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp1kc1u1TAQhS0EoreFB2CDIjasXGwnjm0WSFXVQqVKVAjWlpNMblwldrATpO54B96wT9K53FJ-JDa25DnnG88cQl5wdswZU28yY6IylHFOmaprWj0iG16VgnLG1WOywVNSJRU_IIc5XzNWSc71U3IgBCtLrcyGrFeDy5ALF7qii5PzIRc-FHkefNjG6QZGH26__2iHOEJeIMWxmGBqkguQ3xZ5SWu7rAl--ucUZ0iLR9ya0V6cXX1Ckg90dM0OtEXvMsQuPyNPejdmeH5_H5Ev52efTz_Qy4_vL05PLmkry2qhrgXTN2BAGcnqDqAXrmOi1_gMlW5r1UstS2e0rhstKlX2onG1VL3pQNS6PCLv9tx5bSboWghLcqOdk59curHReft3JfjBbuM3WwrFcJMIeH0PSPHrihuwk88tjCPOH9dsDTe1xCgkKl_9o7yOawo4HYq0KXVdCRTxvahNMecE_cNXOLO7SO0-UouR2l2ktkLPyz9neHD8yhAFYi_IWApbSL87_596BwIxsOI</recordid><startdate>20120201</startdate><enddate>20120201</enddate><creator>Mainali, Laxman</creator><creator>Raguz, Marija</creator><creator>Subczynski, Witold K.</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120201</creationdate><title>Phases and domains in sphingomyelin–cholesterol membranes: structure and properties using EPR spin-labeling methods</title><author>Mainali, Laxman ; Raguz, Marija ; Subczynski, Witold K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c534t-ace9fbe9e79506deef2ad02f8e9fe48c67f5853a9886b82473f2ba657f9de2683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Biochemistry</topic><topic>Biological and Medical Physics</topic><topic>Biomedical and Life Sciences</topic><topic>Biophysics</topic><topic>Cell Biology</topic><topic>Cell Membrane - chemistry</topic><topic>Cholesterol</topic><topic>Cholesterol - chemistry</topic><topic>Electron Spin Resonance Spectroscopy</topic><topic>Hydrophobic and Hydrophilic Interactions</topic><topic>Life Sciences</topic><topic>Lipids</topic><topic>Membrane Biology</topic><topic>Membrane Fluidity</topic><topic>Membranes</topic><topic>Nanotechnology</topic><topic>Neurobiology</topic><topic>Original Paper</topic><topic>Scientific method</topic><topic>Sphingomyelins - chemistry</topic><topic>Spin Labels</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mainali, Laxman</creatorcontrib><creatorcontrib>Raguz, Marija</creatorcontrib><creatorcontrib>Subczynski, Witold K.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Databases</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest research library</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>European biophysics journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mainali, Laxman</au><au>Raguz, Marija</au><au>Subczynski, Witold K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phases and domains in sphingomyelin–cholesterol membranes: structure and properties using EPR spin-labeling methods</atitle><jtitle>European biophysics journal</jtitle><stitle>Eur Biophys J</stitle><addtitle>Eur Biophys J</addtitle><date>2012-02-01</date><risdate>2012</risdate><volume>41</volume><issue>2</issue><spage>147</spage><epage>159</epage><pages>147-159</pages><issn>0175-7571</issn><eissn>1432-1017</eissn><abstract>EPR spin-labeling methods were used to investigate the order and fluidity of alkyl chains, the hydrophobicity of the membrane interior, and the order and motion of cholesterol molecules in coexisting phases and domains, or in a single phase of fluid-phase cholesterol/egg-sphingomyelin (Chol/ESM) membranes with a Chol/ESM mixing ratio from 0 to 3. A complete set of profiles for these properties was obtained for the liquid-disordered (
l
d
) phase without cholesterol, for the liquid-ordered (
l
o
) phase for the entire region of cholesterol solubility in this phase (from 33 to 66 mol%), and for the
l
o
-phase domain that coexists with the cholesterol bilayer domain (CBD). Alkyl chains in the
l
o
phase are more ordered than in the
l
d
pure ESM membrane. However, fluidity in the membrane center is greater. Also, the profile of hydrophobicity changed from a bell to a rectangular shape. These differences are enhanced when the cholesterol content of the
l
o
phase is increased from 33 to 66 mol%, with clear brake-points between the C9 and C10 positions (approximately where the steroid-ring structure of cholesterol reaches into the membrane). The organization and motion of cholesterol molecules in the CBD are similar to those in the
l
o
-phase domain that coexists with the CBD.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>22033879</pmid><doi>10.1007/s00249-011-0766-4</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0175-7571 |
| ispartof | European biophysics journal, 2012-02, Vol.41 (2), p.147-159 |
| issn | 0175-7571 1432-1017 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3270143 |
| source | Springer Nature |
| subjects | Biochemistry Biological and Medical Physics Biomedical and Life Sciences Biophysics Cell Biology Cell Membrane - chemistry Cholesterol Cholesterol - chemistry Electron Spin Resonance Spectroscopy Hydrophobic and Hydrophilic Interactions Life Sciences Lipids Membrane Biology Membrane Fluidity Membranes Nanotechnology Neurobiology Original Paper Scientific method Sphingomyelins - chemistry Spin Labels |
| title | Phases and domains in sphingomyelin–cholesterol membranes: structure and properties using EPR spin-labeling methods |
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