Organization and expression of cloned histone gene clusters from Xenopus laevis and X. borealis

We have isolated several clones containing Xenopus histone genes from genomic libraries of X. laevis and X. borealis DNA. Each genomic clone has been mapped and the positions of 26 histone genes in seven laevis clones and 5 histone genes in one borealis clone have been determined. In laevis, the his...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 1982-12, Vol.10 (23), p.7561-7580
Main Authors: Old, R W, Woodland, H R, Ballantine, J E, Aldridge, T C, Newton, C A, Bains, W A, Turner, P C
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Cell Nucleus - metabolism
Cloning, Molecular
DNA Restriction Enzymes
DNA, Recombinant - metabolism
DNA, Viral - genetics
Female
Genes
Histones - genetics
Microinjections
Oocytes - metabolism
Ovum - metabolism
Protein Biosynthesis
Species Specificity
Xenopus
Xenopus borealis
Xenopus laevis
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have isolated several clones containing Xenopus histone genes from genomic libraries of X. laevis and X. borealis DNA. Each genomic clone has been mapped and the positions of 26 histone genes in seven laevis clones and 5 histone genes in one borealis clone have been determined. In laevis, the histone gene clusters show considerable variation in gene order within a single individual. When the cloned DNAs were microinjected into the nucleus of Xenopus oocytes, expression of cloned genes at the transcriptional and translational level was readily detectable. Previously unknown histone variants were revealed by the microinjection experiments.
ISSN:0305-1048
1362-4962