Frequencies of SLC44A2 alleles encoding human neutrophil antigen-3 variants in the African American population

BACKGROUND: The human neutrophil antigen‐3 (HNA‐3) epitopes reside on the choline transporter‐like protein‐2 (CTL2). A single‐nucleotide substitution (461G>A; Arg154Gln) on the CTL2 gene (SLC44A2) defines the allele SLC44A2*1, which expresses HNA‐3a, and SLC44A2*2, which expresses HNA‐3b; an addi...

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Published in:Transfusion (Philadelphia, Pa.) Pa.), 2012-05, Vol.52 (5), p.1106-1111
Main Authors: Huvard, Michael J., Schmid, Pirmin, Stroncek, David F., Flegel, Willy A.
Format: Article
Language:English
Subjects:
African Americans - genetics
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Biological and medical sciences
Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis
European Continental Ancestry Group - genetics
Female
Fundamental and applied biological sciences. Psychology
Gene Frequency
Humans
Isoantigens - genetics
Isoantigens - immunology
Male
Medical sciences
Membrane Glycoproteins - genetics
Membrane Transport Proteins - genetics
Polymorphism, Genetic
Transfusions. Complications. Transfusion reactions. Cell and gene therapy
Vertebrates: blood, hematopoietic organs, reticuloendothelial system
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Summary:BACKGROUND: The human neutrophil antigen‐3 (HNA‐3) epitopes reside on the choline transporter‐like protein‐2 (CTL2). A single‐nucleotide substitution (461G>A; Arg154Gln) on the CTL2 gene (SLC44A2) defines the allele SLC44A2*1, which expresses HNA‐3a, and SLC44A2*2, which expresses HNA‐3b; an additional substitution (457C>T; Leu153Phe) in SLC44A2*1:2 may impact genotyping systems. People who only express HNA‐3b may develop anti‐HNA‐3a. These alloantibodies have been linked to severe transfusion‐related acute lung injury, which may be a reason to screen blood donors for SLC44A2*2 homozygosity. For Caucasian and Asian populations, SLC44A2 allele frequencies are known. Our primary objective was to determine the SLC44A2 allele frequencies in the African American population. STUDY DESIGN AND METHODS: Purified DNA from 334 individuals (202 male, 132 female; 241 African American, 93 Caucasian) was collected. Two real‐time polymerase chain reaction assays were developed to genotype all samples; results were confirmed by nucleotide sequencing. RESULTS: In 241 African American donors, the allele frequency of SLC44A2*1 was 93% (85%‐
ISSN:0041-1132
1537-2995
DOI:10.1111/j.1537-2995.2011.03396.x