A short half-life GFP mouse model for analysis of suprachiasmatic nucleus organization

Period1 ( Per1) is one of several clock genes driving the oscillatory mechanisms that mediate circadian rhythmicity. Per1 mRNA and protein are highly expressed in the suprachiasmatic nuclei, which contain oscillator cells that drive circadian rhythmicity in physiological and behavioral responses. We...

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Bibliographic Details
Published in:Brain research 2003-02, Vol.964 (2), p.279-287
Main Authors: LeSauter, Joseph, Yan, Lily, Vishnubhotla, Bhavana, Quintero, Jorge E, Kuhlman, Sandra J, McMahon, Douglas G, Silver, Rae
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Cycle Proteins
Chronobiology
Circadian rhythm
Circadian Rhythm - genetics
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Green fluorescent protein
Green Fluorescent Proteins
Half-Life
Immunohistochemistry
In Situ Hybridization
Light
Locomotion
Luminescent Proteins
Mice
Mice, Transgenic
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Period Circadian Proteins
Period gene
Promoter Regions, Genetic
RNA, Messenger - metabolism
Suprachiasmatic nucleus
Suprachiasmatic Nucleus - metabolism
Transcription Factors
Transgenic
Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:Period1 ( Per1) is one of several clock genes driving the oscillatory mechanisms that mediate circadian rhythmicity. Per1 mRNA and protein are highly expressed in the suprachiasmatic nuclei, which contain oscillator cells that drive circadian rhythmicity in physiological and behavioral responses. We examined a transgenic mouse in which degradable green fluorescent protein (GFP) is driven by the mPer1 gene promoter. This mouse expresses precise free-running rhythms and characteristic light induced phase shifts. GFP protein (reporting Per1 mRNA) is expressed rhythmically as measured by either fluorescence or immunocytochemistry. In addition the animals show predicted rhythms of Per1 mRNA, PER1 and PER2 proteins. The localization of GFP overlaps with that of Per1 mRNA, PER1 and PER2 proteins. Together, these results suggest that GFP reports rhythmic Per1 expression. A surprising finding is that, at their peak expression time GFP, Per1 mRNA, PER1 and PER2 proteins are absent or not detectable in a subpopulation of SCN cells located in the core region of the nucleus.
ISSN:0006-8993
1872-6240
DOI:10.1016/S0006-8993(02)04084-2